Biolegend, 379702, Purified anti-human ATG3 Antibody, 100μg

Human autophagy related 3, ATG3, is a 314 amino acid protein with a molecular mass of 36 kD, which is a ubiquitin-like modifier similar to the canonical E2 enzyme. ATG3 is a widely expressed conjugating enzyme for APG8 lipidation, an essential step for the initiation of autophagy. Autophagy is the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway which plays an important role in controlling normal growth but may be defective in tumor cells. ATG3 is essential for autophagocytosis, which is a starvation-induced process responsible for transport of cytoplasmic proteins to the lysosome/vacuole.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2922609 (BioLegend Cat. No. 379702)
Structure: Human ATG3 is a 314 amino acid protein with a molecular mass of 36 kD
Distribution: Expressed in testis and bone marrow stromal cell
Function: E2-conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2, or MAP1LC3A).
Interaction: Interacts with ATG7 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5.
Ligand/Receptor: ATG8
Biology Area: Cell Biology, Cell Death, Immunology
Molecular Family: Apolipoproteins, Autophagosome Markers
Antigen References: Isei T, et al. 2002. J Biol Chem. 277:13739. Silke M. et al. 2009. Hum Genet. 128:453. Ozlem O, et al. 2012. Apoptosis. 17:810.
Gene ID: 64422
UniProt: View information about ATG3 on UniProt.org
Clone: A19237B
Regulatory Status: RUO
Other Names: Autophagy Related 3
Isotype: Mouse IgG2a, κ