Biolegend, 380502, Purified anti-human CD209/CD299 (DC-SIGN/L-SIGN) Antibody, 100μg

CD209 (DC-SIGN) is a C-type lectin that has a high affinity for the ICAM3 molecule. It binds various microorganisms by recognizing high mannose-containing glycoproteins on their envelopes. It especially functions as a receptor for several viruses such as HIV and Hepatitis C. CD299 (L-SIGN) is a transmembrane receptor and is referred to as L-SIGN because of its expression in the endothelial cells of the lymph nodes and liver. The protein is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain, and a C-type lectin carbohydrate recognition domain.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human CD209 protein extracellular domain
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone A20120F can completely block the binding of clone 14E3G7 to target cells. It does not affect the binding of clone A20120B, clone 9E9A8 and clone DCS-8C1.
RRID: AB_2924572 (BioLegend Cat. No. 380502)
Structure: DC-SIGN is a 44 kD type II transmembrane glycoprotein and a member of the C-type lectin family. DC-SIGNR (DC-SIGN-related molecule) is a type II integral membrane protein that is 77% identical to DC-SIGN.
Distribution: Dendritic cells, endothelial cells, macrophages
Function: CD299 mediates interactions between dendritic cells and resting T cells and has a high affinity for HIV-1 gp120. CD209 plays a critical role in capturing and internalizing pathogens. LSP1 (leukocyte-specific protein 1) interacts with the cytoplasmic domain of CD209 and mediates the transport of HIV to the proteasome.
Ligand/Receptor: CD209 binds to ICAM-3 (CD50), ICAM-2 (CD102), and Butyrophilin (BTNA2A1). CD299 is a receptor for ICAM-3 (CD50).
Cell Type: Dendritic cells, Endothelial cells, Macrophages
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules
Antigen References: Fauci AS. 1996. Nature. 384:529-34. Yokoyama-Kobayashi M, et al. 1999. Gene. 228:161-7. Soilleux EJ, et al. 2000. J Immunol. 165:2937-42. Pohlmann S, et al. 2001. Proc. Natl. Acad. Sci. USA 98:2670-5. Bashirova AA, et al. 2001. J Exp Med. 193:671-8. Lai WK, et al. 2006. Am J Pathol. 169:20-8.
Gene ID: 3083510332
UniProt: View information about CD209/CD299 on UniProt.org
Clone: A20120F
Regulatory Status: RUO
Other Names: For CD209: CDSIGN, CLEC4L, DC-SIGN, DC-SIGN1, For CD299: LSIGN, CD209L, L-SIGN, DCSIGNR, HP10347, DC-SIGN2, DC-SIGNR
Isotype: Rat IgG2a, κ