Biolegend, 381502, Purified anti-Puromycin Antibody, 100μg

Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, which functions as a Tyr-tRNA mimetic that enters the ribosome A site and incorporates into the nascent chain C terminus. Antibodies against Puromycin can detect puromycylated nascent chains released from ribosomes and thereby quantify protein translation. This monoclonal antibody to puromycin can be used in flow cytometry, immunofluorescence or immunoblotting to measure rates of global protein synthesis (mRNA translation) in cells incubated with Puromycin.
100μg
Verified Reactivity: Human, Mouse, Rat, All species
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Puromycin from Streptomyces alboniger coupled to KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ICFC - Quality tested ICC, WB, IP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration of 2.5 μg/mL is recommended. For western blotting, the suggested use of this reagent is 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.0 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: It is recommended that the timing and concentration of Puromycin treatment be optimized for each cell type. 1 - 10 µg/mL of Puromycin for 30 minutes to 2 hours are suggested ranges for activated PBMCs or cell lines in growth phase. For flow cytometry, the amount of anti-puromycin antibody may also have to be optimized. Cells with high levels of protein translation require lower concentrations of antibody.
RRID: AB_2924575 (BioLegend Cat. No. 381502)
Distribution: Cytoplasm, Ribosomes
Cell Type: Embryonic Stem Cells
Biology Area: Cell Biology, Protein Synthesis
Antigen References: Schmidt E, et al. 2009. Nat Methods. 6:275-7. Arguello R, et al. 2020. Cell Metabolism. 32:1063-1075.
Gene ID: NA
UniProt: View information about Puromycin on UniProt.org
Clone: 2A4
Regulatory Status: RUO
Other Names: puromycin from streptomyces alboniger
Isotype: Mouse IgG2a, κ