Biolegend, 382108, PE anti-human CD157 (BST-1) Antibody, 100tests

CD157, also known as Bone Marrow Stromal Cell Antigen 1 (BST1), is a GPI-anchored glycoprotein. It is an ectoenzyme that has both cyclic ADP-ribose hydrolase and ADP-ribosyl cyclase activities. It is prevalently expressed by cells of the myelomonocytic lineage, and modulates adhesion of neutrophils and monocytes to the extracellular matrix. CD157 is expressed in 97% of primary acute myeloid leukemia samples, and is used as a marker for diagnosis of Paroxysmal Nocturnal Hemoglobinuria, a disease caused by intrinsic deficiency of GPI anchors.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: hCD157 stable transfectant followed by recombinant protein (aa29-293)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_2927935 (BioLegend Cat. No. 382107) AB_2927935 (BioLegend Cat. No. 382108)
Structure: GPI-anchored, ADP-ribosyl cyclase
Distribution: Cell Membrane
Function: ADP-ribosyl cyclase activity. Fibronectin, fibrinogen, laminin-1 and type 1 collagen binding. Hydrolase, Transferase.
Interaction: CD11b/CD18, ECM components
Ligand/Receptor: NAD, NADP
Cell Type: Antigen-presenting cells, Granulocytes, Monocytes
Biology Area: Cell Adhesion, Cell Proliferation and Viability, Innate Immunity
Molecular Family: CD Molecules, Enzymes and Regulators, Innate Immune Signaling
Antigen References: Ortolan E, et al. 2019. Immunology Letters. 205:59-64 Ferrero E, et al. 2017. Sci Rep. 7: 15923. Kaisho T, et al. 1994. Proc Natl Acad Sci USA. 91:5325-9. Morone S, et al. 2014. J Biol Chem. 289:15588-601.
Gene ID: 683
UniProt: View information about CD157 on UniProt.org
Clone: W21007F
Regulatory Status: RUO
Other Names: BST1
Isotype: Rat IgG2a, λ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.