Biolegend, 383208, PE anti-human CD277 (BTN3A) Recombinant Antibody, 100tests

Butyrophilin subfamily 3A (BTN3A), also known as CD277, is a 56 kD type 1 transmembrane glycoprotein. The butyrophilin (BT)/B7 3A family includes three members, BTN3A1, BTN3A2, and BTN3A3, which share a highly conserved extracellular IgV-like domain. BTN3A is broadly expressed in various immune cells including T cells, B cells, NK cells, monocytes, dendritic cells, endothelial cells, and some tumor cells. It plays a role in T cell activation, regulating their proliferation and release of IFNƳ and other cytokines. Clone 34-7.Rec has been shown to bind to a V region of BTN3A3 that is shared with BTN3A1 and BTN3A2.
100tests
Verified Reactivity: Human
Antibody Type: Recombinant
Host Species: Mouse
Immunogen: pRC-CMV-BTN3A3
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: This product was formerly named “anti-human CD277 (BTN3A1).” The 34-7.Rec clone has been confirmed to recognize BTN3A3. Cross-reactivity with other BTN3A subfamily members has not been tested.
RRID: AB_3097513 (BioLegend Cat. No. 383207) AB_3097513 (BioLegend Cat. No. 383208)
Structure: 56 kD single pass type I membrane glycoprotein, member of the immunoglobin superfamily
Distribution: Cell membrane
Function: Signaling receptor binding, Homodimerization
Interaction: TFF1, PEPL, BT3A2, G5E9A7
Biology Area: Immuno-Oncology, Inhibitory Molecules
Molecular Family: CD Molecules, Tumor Suppressors
Antigen References: Vantourout P, et al. 2018. Proc Natl Acad Sci USA. 115:1039-44. Payne KK, et al. 2020. Science. 369: 942-9. Rigau M, et lat. 2020. Science. 367:eaay5516. Yang Y, et al. 2019. Immunity. 50:1043-53.
Gene ID: 1111910384
UniProt: View information about CD277 on UniProt.org
Clone: 34-7.Rec
Regulatory Status: RUO
Other Names: BTF5, BT3.1, BTN3.1, BT3.2, BTF4, BTN3.2, BTF3, BTN3.3
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.