Biolegend, 384206, PE anti-human Integrin αVβ6 Antibody, 100tests

Integrins are heterodimeric transmembrane receptors comprising alpha and beta subunits that function as cell anchoring and signaling molecules. The ITGAV gene encodes integrin alpha V, a type I integral membrane glycoprotein known as the vitronectin receptor α chain, or CD51. It forms a heterodimer with integrin β1 (CD29), β3 (CD61), β5, β6, or β8. Integrin αV contains two disulfide-linked subunits of 125 kD and 24 kD, and is expressed on endothelial cells, fibroblasts, macrophages, platelets, osteoclasts, neuroblastoma, melanoma, and hepatoma cells. Integrin beta 6 is a protein that in humans is encoded by the ITGB6 gene. The β6 subunit only combines with αV to generate a single heterodimer. Integrin αVβ6 is a subtype of the integrin family expressed exclusively on epithelial cells. Integrin αVβ6 is usually expressed at low or undetectable levels in normal adult tissues but can be highly upregulated during pathological and physiological processes such as wound healing, fibrosis, inflammation, and cancer.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Marsh D, el al. 2008. Cancer Res. 68:3295.
RRID: AB_3106248 (BioLegend Cat. No. 384205) AB_3106248 (BioLegend Cat. No. 384206)
Distribution: Epithelial cells
Function: Modulate innate immune surveillance, maintain epithelial stem cell quiescence
Ligand/Receptor: Serve as a fibronectin receptor
Cell Type: Epithelial cells
Biology Area: Angiogenesis, Cell Adhesion
Molecular Family: Adhesion Molecules
Gene ID: 36853694
UniProt: View information about Integrin alphaVbeta6 on UniProt.org
Clone: 53a.2
Regulatory Status: RUO
Other Names: Integrin alpha V beta 6
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.