Biolegend, 384402, Purified anti-human CD45 Recombinant Antibody, 100μg

CD45 is a 180 - 240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes or platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.
100μg
Verified Reactivity: Human
Antibody Type: Recombinant
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2927952 (BioLegend Cat. No. 384402)
Structure: Type I transmembrane protein, 180 – 240 kD (multiple isoforms)
Distribution: Hematopoietic cells, except circulating erythrocytes or platelets
Function: Tyrosine phosphatases, signaling
Cell Type: B cells, Granulocytes, Leukocytes, Lymphocytes, Monocytes, T cells
Biology Area: Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Thomas M. 1989. Annu Rev Immunol. 7:339-69. Trowbridge I, et al. 1994. Annu Rev Immunol. 12:85-116.
Gene ID: 5788
UniProt: View information about CD45 on UniProt.org
Clone: QA21A24
Regulatory Status: RUO
Other Names: CD45, Leukocyte Common Antigen (LCA), T200
Isotype: Mouse IgG1, κ