Biolegend, 385702, Purified anti-human CD69 Recombinant Antibody, 100μg

CD69, also known as activation inducer molecule (AIM), very early activation antigen (VEA), and MLR3, is a 27-33 kD type II transmembrane protein. It is a member of the C-type lectin family and expressed as a disulfide-linked homodimer. Other members of this receptor family include NKG2, NKR-P1 CD94, and Ly49. CD69 is transiently expressed on activated leukocytes including T cells, thymocytes, B cells, NK cells, neutrophils, and eosinophils. CD69 is constitutively expressed by a subset of medullary mature thymocytes, platelets, mantle B cells, and certain CD4+ T cells in germinal centers of normal lymph nodes. CD69 is involved in early events of lymphocyte, monocyte, and platelet activation, and has a functional role in redirected lysis mediated by activated NK cells.
100μg
Verified Reactivity: Human
Antibody Type: Recombinant
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone QA18A26 can completely block the binding of clone FN50 on target cells.
RRID: AB_2927999 (BioLegend Cat. No. 385702)
Structure: C-type lectin, type II glycoprotein, 28/32 kD
Distribution: Activated T cells, B cells, NK cels, Granulocytes, thymocytes, platelets, Langerhans cells
Function: Lymphocyte, monocyte, and platelet activation, NK cell killing
Cell Type: B cells, Lymphocytes, NK cells, Platelets, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. Marzio R, et al. 1999. Immunopharmacology and immunotoxicology. 21:565-82.
Gene ID: 969
UniProt: View information about CD69 on UniProt.org
Clone: QA18A26
Regulatory Status: RUO
Other Names: Very Early Activation Antigen (VEA), Activation inducer molecule (AIM)
Isotype: Mouse IgG1, κ