Biolegend, 386902, Purified anti-GATA3 Antibody, 100μg

GATA3 functions as a major regulator of T helper 2 (Th2) cell differentiation in immune cells. GATA3 expression is upregulated through IL-4 receptor signaling or the Notch-mediated pathway, which results in production of IL-4, IL-5, and IL-13 cytokines. This is responsible for the preferential differentiation of Th2-cells. GATA3 has been shown to regulate early developmental processes of T cells, such as T cell commitment, positive selection, and post-commitment CD4+ T cell survival. In the mammary gland, GATA3 plays an important role in differentiation and commitment of luminal epithelial cells. In a mouse model, exogenous expression of GATA3 in undifferentiated breast carcinoma cells induces differentiation and suppresses spreading of the tumor cells, suggesting that GATA3 is involved in preventing malignancy of breast cancer. GATA3 has been reported to be essential in the development of a variety of normal tissues. Defects in GATA3 result in hypoparathyroidism, sensorineural deafness, and renal dysplasia (HDR syndrome).
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial human GATA3 recombinant protein (1-258 aa)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone W19195B does not block 16E10A23, TWAJ, or L50-823.
RRID: AB_2941570 (BioLegend Cat. No. 386902)
Structure: 48 kD protein containing two GATA-type zinc finger domains responsible for DNA binding and two trans-activating domains at N-terminus
Distribution: Nucleus
Function: GATA3, a transcriptional activator, binds to a consensus DNA sequence 5'-(A/T) GATA(A/G)-3' in the promoter region of genes, regulates T cell development, TH2 cell differentiation, and luminal cell proliferation
Interaction: Interacts with Smad3, FOG1, FOG2, and PU.1
Biology Area: Cell Biology, Chromatin Remodeling/Epigenetics, Immunology, Signal Transduction, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: Yagi R, et al. 2011. Int Immunol. 23:415-20. Chou J, et al. 2010. J Cell Physiol. 222:42-9. Ho IC, et al. 2009. Nat Rev Immunol. 9:125-35. Rothenberg EV, et al. 2008. Semin Immunol. 20:236-46. Kouros-Mehr H, et al. 2008. Curr Opin Cell Biol. 20:164-70. Van Esch H, et al. 2000. Nature. 406:419-22.
Gene ID: 2625
UniProt: View information about GATA3 on UniProt.org
Clone: W19195B
Regulatory Status: RUO
Other Names: GATA-binding factor 3, trans-acting T-cell-specific transcription factor GATA-3
Isotype: Rat IgG2a, κ