Biolegend, 387904, PE anti-human CD178 (FasL) Antibody, 100tests

CD178, also known as Fas ligand and CD95L, is a 38-42 kD type II glycoprotein. CD178 belongs to the TNF superfamily and is expressed on activated T lymphocytes, NK cells, monocytes, and granulocytes. CD178 is also expressed on parenchymal cells of the retina and cornea, retinal pigment epithelial cells, and testis. The extracellular region of FasL can be cleaved by matrix metalloproteinases (MMPs) to give rise to a 26 kD soluble protein. CD178 binds to CD95, a member of the TNFR superfamily, to induce apoptosis. CD95/CD95L interactions play an important role in the maintenance of peripheral tolerance and survival.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: L5178Y mouse T lymphoma cells expressing recombinant human FasL
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_3675115 (BioLegend Cat. No. 387903) AB_3675115 (BioLegend Cat. No. 387904)
Structure: TNF superfamily, type II glycoprotein, 38-42 kD, 26 kD soluble form
Distribution: Activated T cells, NK cells, testis, eye, neutrophils, Clara type II cells
Function: Apoptosis/Cell Death
Interaction: The interaction of Fas Ligand and FasR on an adjacent cell leads to the trimerization and formation of the death-inducing signaling complex (DISC).
Ligand/Receptor: FASR (CD95)
Cell Type: Neutrophils, NK cells, T cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience
Molecular Family: CD Molecules
Antigen References: Suda T, et al. 1997. J Exp Med. 186:2045-50. Kayagaki N, et al. 1995. J Exp Med. 182:1777-83. Tanaka M, et al. 1995. EMBO J. 14:1129-35.
Gene ID: 356
UniProt: View information about CD178 on UniProt.org
Clone: W19402J
Regulatory Status: RUO
Other Names: Fas Ligand (FasL), CD95L, TNFSF6, Fas-L
Isotype: Rat IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.