Biolegend, 388002, Purified anti-human CD194 (CCR4) Recombinant Antibody, 100μg

CD194, also known as CCR4, is a CC chemokine receptor. It binds CCL17 and CCL22 and is expressed on a subset of T and B cells, basophils, monocytes, and NK cells. Human Th2 cells are characterized by the expression of CCR4 and CCR8, and these receptors are regulated differently during Th2 development. Human peripheral blood Tregs can be divided into two distinct populations based on the expression of CCR4. Freshly isolated Tregs express CCR4 and presumably represent memory-type Tregs, and CCR4- Tregs require CD3-mediated activation to acquire a regulatory activity. Depletion of CCR4+ T cells leads to Th1-type polarization of CD4+ T cells and augmentation of CD8+ T cell responses to tumor antigens. CCR4 and its ligands are important for the recruitment of memory T cells into the skin in various cutaneous immune diseases.
100μg
Verified Reactivity: Human
Antibody Type: Recombinant
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone QA21A43 can completely block the binding of clone L291H4 on target cells.
RRID: AB_3068206 (BioLegend Cat. No. 388002)
Structure: GPCR, seven transmembrane receptor
Distribution: Subsets of T and B cells, basophils, monocytes, NK cells
Function: Migration of inflammatory and regulatory cells to the target tissues
Ligand/Receptor: CCL17 and CCL22
Cell Type: B cells, Basophils, Embryonic Stem Cells, Monocytes, NK cells, T cells, Tregs
Biology Area: Immunology, Innate Immunity, Stem Cells
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: Katschke KJ, et al. 2001. Arthritis Rheum. 44:1022-32. Colantonio L, et al. 2002. Eur J Immunol. 32:1264-73. Jakubzick C, et al. 2004. Am J Pathol. 165:1211-21. Morimoto Y, et al. 2005. J Leukoc Biol. 78:753-61. Baatar D, et al. 2007. J Immunol. 178:4891-900. Kusumoto M, et al. 2007. J Interferon Cytokine Res. 27:901-10.
Gene ID: 1233
UniProt: View information about CD194 on UniProt.org
Clone: QA21A43
Regulatory Status: RUO
Other Names: CKR4, K5-5, CMKBR4, ChemR13, CC-CKR-4, MGC88293, HGCN:14099
Isotype: Mouse IgG1, κ