Biolegend, 388204, PE anti-human CD154 (CD40L) Antibody, 100tests

CD154, also known as CD40L, TNFSF5, T-BAM, and gp39, is a 32-39 kD type II transmembrane glycoprotein member of the TNF superfamily expressed on activated T cells. CD154 interacts as a costimulatory molecule with CD40 on B Cells to induce B cell activation, resulting in proliferation, immunoglobulin production, and Ig Isotype switching. CD40L also may regulate T cell cytokine production.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human CD40L protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone W20199I is able to block the binding of clone 24-31 on target cells. Clone W20199I is able to block the binding of CD40 and CD40L interaction on target cells.
RRID: AB_3105982 (BioLegend Cat. No. 388203) AB_3105982 (BioLegend Cat. No. 388204)
Structure: 32-39 kD type II transmembrane glycoprotein of the TNF superfamily
Distribution: Activated T cells
Function: B cell costimulation
Ligand/Receptor: CD40
Cell Type: T cells, Tregs
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: van Kooten C and Banchereau J. 2000. J Leukoc Biol. 67:2-17. Ford GS, et al. 1999. J Immunol. 162:4037-44.
Gene ID: 959
UniProt: View information about CD154 on UniProt.org
Clone: W20199I
Regulatory Status: RUO
Other Names: CD40L, TNFSF5, T-BAM, gp39
Isotype: Rat IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.