Biolegend, 390804, Purified anti-human/mouse Galectin-9 Antibody, 100μg

Galectin-9 is a mammalian lectin with a molecular weight around 50 kD. It is a member of the β-galactoside-binding family. With two conserved carbohydrate recognition domains (CRDs), galectin-9 binds small β-galactosides as well as complex glycoconjugates. HAVCR2/TIM3 has been reported as one of its ligands. Galectin-9 may be retained intracellularly or transported to the cell surface where it can be cleaved to generate a soluble form. Galectin-9 is expressed by lymphocytes, dendritic cells, granulocytes, eosinophils, astrocytes, endothelial cells, fibroblasts, and thymus epithelial cells. It can be induced by cytokines in various cell types and is involved in cell aggregation, adhesion, chemotaxis, and apoptosis. Galectin-9 induces regulatory T cells and suppresses Th1 and Th17 responses.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human galectin-9 protein (a.a 2-323)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ICFC - Quality tested FC, ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our Cyto-Fast™ Fix/Perm Buffer Set. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration of 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance in each application.
Application Notes: Clone W21101F does not block the staining of reference clone 9M1-3. Recombinant protein completely blocks the staining of test clone W21101F.
RRID: AB_3662367 (BioLegend Cat. No. 390804)
Structure: Member of the β-galactoside-binding lectin family. Molecular weight around 50 kD. Binds small β-galactosides as well as complex glycoconjugates with two conserved carbohydrate recognition domains (CRDs).
Distribution: Lymphocytes, dendritic cells, neutrophils, eosinophils, astrocytes, endothelial cells, fibroblasts, thymus stromal/epithelial cells
Function: Cell aggregation, adhesion, chemotaxis, apoptosis, suppression of Th1 and Th17 responses, and induction of regulatory T cells
Interaction: Astrocytes, dendritic cells, endothelial cells, eosinophils, epithelial cells, fibroblasts, lymphocytes, neutrophils, tregs
Ligand/Receptor: HAVCR2/TIM3
Cell Type: Astrocytes, Dendritic cells, Endothelial cells, Eosinophils, Epithelial cells, Fibroblasts, Lymphocytes, Neutrophils, Tregs
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
Molecular Family: Adhesion Molecules, Immune Checkpoint Receptors
Antigen References: Seki M, et al. 2008. Clin Immunol. 127:78-88. Tsuboi Y, et al. 2007. Clin Immunol. 124:221-33. Zhu C, et al. 2005. Nat Immunol. 6:1245-52. Dunphy JL, et al. 2002. J Biol Chem. 277:14916-24.
Gene ID: 8795
UniProt: View information about Galectin-9 on UniProt.org
Clone: W21101F
Regulatory Status: RUO
Other Names: Lgals9, Gal-9, Ecalectin, Tumor antigen HOM-HD-21, Ecalectin
Isotype: Mouse IgG1, κ