Biolegend, 391902, Purified anti-human GPR56 Antibody, 100μg

GPR56, also known as TM7XN1, is an orphan G-protein-coupled receptor (GPCR) containing seven transmembrane domains and a mucin-like domain in the N-terminal region. The total length of the human GPR56 gene is approximately 15 kb, and it consists of 13 exons and encodes 693 amino acids.  GPR56 is expressed in a wide range of tissues, such as brain, thyroid gland, and heart. Mutations in human GPR56 gene were found to cause a specific brain malformation called bilateral frontoparietal polymicrogyria. Recently, GPR56 was found specifically expressed on cytotoxic NK and T lymphocytes, including CD8 + , CD4 + , and γδ T cells, which might affect the migratory properties of these cells. GPR56 was also found significantly downregulated in some cancer cells, suggesting that it may play a role in growth suppression and cancer metastasis.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human GPR56 extracellular domain, complete Freund’s adjuvant, incomplete Freund’s adjuvant
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested Blocking, Direct ELISA, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: blocking the cell migration (1).
Application References(PubMed link indicates BioLegend citation): Ohta S, et al. 2015. Biol. Pharm. Bull. 38:594.
RRID: AB_2687290 (BioLegend Cat. No. 391902)
Structure: Contains seven transmembrane domains and a mucin-like domain in the N-terminal region.
Distribution: T cells, NK cells, wide range of tissues such as brain, thyroid gland, and heart.
Function: May be involved in immune cell migration, cancer cell growth metastasis, and neuron development.
Cell Type: NK cells, T cells
Antigen References: 1. Peng YM, et al. 2011. J. Leuko. Biol. 90:735. 2. Shashidhar S, et al. 2005. Oncogene 24:1673. 3. Piao X, et al. 2004. Science 303:2033. 4. Liu M, et al. 1999. Genomics 55:296.
Gene ID: 9289
UniProt: View information about GPR56 on UniProt.org
Clone: 4C3
Regulatory Status: RUO
Other Names: TM7XN1, ADGRG1
Isotype: Mouse IgG1, κ