Biolegend, 392106, Brilliant Violet 421™ anti-human P2RY12 Antibody, 100tests

P2RY12 is a receptor for ADP and ATP coupled to G-proteins that inhibit the adenylyl cyclase second messenger system and is not activated by UDP and UTP. P2RY12 is required for normal platelet aggregation and blood coagulation, and is a target to inhibit of platelet aggregation in the treatment of thromboembolisms and other clotting disorders. P2RY12 is expressed on platelets, lung, appendix, pituitary and adrenal glands; this molecule is a very useful marker to identify microglial cells in the brain.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human P2RY12-transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: View the video of our collaborator discussing the importance of highly specific monoclonal antibodies for P2RY12 in Microglia research.
Product Citations: Jairaman A, et al. 2022. Elife. 11:. PubMed Yeini E, et al. 2021. Nat Commun. 12:1912. PubMed
RRID: AB_2783290 (BioLegend Cat. No. 392105) AB_2783291 (BioLegend Cat. No. 392106)
Distribution: Platelets, microglia
Function: Inhibit the adenylyl cyclase second messenger system
Ligand/Receptor: ADP, ATP
Cell Type: Microglia, Platelets
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family: GPCR, Purinergic Receptors
Antigen References: 1. Lecchi A, et al. 2015. Blood. 125:1006. 2. Zhang K, et al. 2014. Nature. 509:115. 3. Zhang J, et al. 2014. Nature. 509:119. 4. Cornelissen I, et al. 2010. Proc. Natl. Acad. Sci. USA. 107:18605. 5. Paruchuri S, et al. 2009. J. Exp. Med. 206:2543.
Gene ID: 64805
UniProt: View information about P2RY12 on UniProt.org
Clone: S16001E
Regulatory Status: RUO
Other Names: ADPG-R, BDPLT8, HORK3, P2T(AC), P2Y(12)R, P2Y(AC), P2Y(ADP), P2Y(cyc), P2Y12 platelet ADP receptor, purinergic receptor P2RY12, purinergic receptor P2Y, G-protein coupled
Isotype: Mouse IgG2a, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.