Biolegend, 392502, Purified anti-human CD19 Antibody, 100μg

CD19 is a 95 kD type I transmembrane glycoprotein also known as B4. It is a member of the immunoglobulin superfamily expressed on B-cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human Chronic Lymphocytic Leukemia (CLL) cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone 4G7 partially blocks anti-human CD19 clones HIB19 and SJ25C1 staining based on in-house testing
Application References(PubMed link indicates BioLegend citation): Cabezudo E, et al. 1999. Haematologica. 84:413-8 Van Zelm MC et al. 2010. J Clin Invest. 120:1265-74
Product Citations: Gehlert CL, et al. 2022. Front Immunol. 13:957874. PubMed Nugent JL, et al. 2021. iScience. 24:103421. PubMed Winterberg D, et al. 2021. J Clin Med. 10:. PubMed
RRID: AB_2721738 (BioLegend Cat. No. 392502)
Structure: Ig superfamily, type I transmembrane glycoprotein, 95 kD
Distribution: Normal and malignant B lymphocytes
Function: B cell activation and differentiation
Ligand/Receptor: Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR coreceptor
Cell Type: B cells, Lymphocytes
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: Tedder T, et al. 1994. Immunol. Today 15:437. Bradbury L, et al. 1993. J. Immunol. 151:2915.
Gene ID: 930
UniProt: View information about CD19 on UniProt.org
Clone: 4G7
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: B4, CVID3
Isotype: Mouse IgG1, κ