Biolegend, 392508, FITC anti-human CD19 Antibody, 100tests

CD19 is a 95 kD type I transmembrane glycoprotein also known as B4. It is a member of the immunoglobulin superfamily expressed on B-cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human Chronic Lymphocytic Leukemia (CLL) cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Clone 4G7 partially blocks anti-human CD19 clones HIB19 and SJ25C1 staining based on in-house testing
Application References(PubMed link indicates BioLegend citation): Cabezudo E, et al. 1999. Haematologica. 84:413-8 Van Zelm MC et al. 2010. J Clin Invest. 120:1265-74
Product Citations: Xin Y, et al. 2021. Stem Cell Res Ther. 49:12. PubMed Doloff JC, et al. 2023. Sci Adv. 9:eade9488. PubMed Zhang Y, et al. 2022. J Immunol Res. 2022:9003902. PubMed Cao Y, et al. 2022. Cell Rep. 41:111845. PubMed Wu L, et al. 2023. Cell Rep Med. 4:100917. PubMed Tu S, et al. 2020. Cellular Signalling. 73:109695. PubMed Cao Y, et al. 2022. Nature. :. PubMed Cao Y, et al. 2022. Nature. 602:657. PubMed Riquelme SA, et al. 2020. Cell Metabolism. 31(6):1091-1106.e6. PubMed Cao Y, et al. 2021. Cell Res. 31:732. PubMed Liu Y, et al. 2019. Dis Markers. 2019:2407067. PubMed He Z, et al. 2020. J Exp Clin Cancer Res. 39:140. PubMed Li K, et al. 2020. Nat Commun. 4.844444444. PubMed Vanoni G, et al. 2021. eLife. 10:00. PubMed
RRID: AB_2750098 (BioLegend Cat. No. 392507) AB_2750099 (BioLegend Cat. No. 392508)
Structure: Ig superfamily, type I transmembrane glycoprotein, 95 kD
Distribution: Normal and malignant B lymphocytes
Function: B cell activation and differentiation
Ligand/Receptor: Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR coreceptor
Cell Type: B cells, Lymphocytes
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: Tedder T, et al. 1994. Immunol. Today 15:437. Bradbury L, et al. 1993. J. Immunol. 151:2915.
Gene ID: 930
UniProt: View information about CD19 on UniProt.org
Clone: 4G7
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: B4, CVID3
Isotype: Mouse IgG1, κ