Biolegend, 392918, PE/Fire™ 640 anti-human CD66b Antibody, 100tests

CD66b is a 95-100 kD glycosylphosphatidylinositol (GPI)-linked protein also known as CD67, CGM6, and NCA-95. CD66b is a member of the immunoglobulin superfamily, carcinoembryonic antigen (CEA)-like subfamily. CD66b, expressed on granulocytes, has been reported to induce activation in neutrophils and to be involved in heterophilic adhesion with CD66c.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant soluble human CEACAM8-Fc produced in HEK293 cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 640 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * PE/Fire™ 640 has a maximum excitation of 566 nm and a maximum emission of 639 nm.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Singer, et al. 2014. PLoS One. 9(4):e94106. PMID: 24743304 Klaile, et al. 2013. Respir Res. 14:85. PMID 23941132
RRID: AB_2876709 (BioLegend Cat. No. 392917) AB_2876709 (BioLegend Cat. No. 392918)
Structure: Ig superfamily, CEA antigen group, GPI-linked glycoprotein
Distribution: Granulocytes
Function: Cell adhesion, neutrophil activation
Ligand/Receptor: CD66c
Cell Type: Granulocytes
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Kuijpers T, et al. 1993. J Immunol. 151:4934. Kuroki M, et al. 1992. J Leuk Biol. 52:551.
Gene ID: 1088
UniProt: View information about CD66b on UniProt.org
Clone: 6/40c
Regulatory Status: RUO
Other Names: CEACAM8, CD67, CGM6, NCA-95
Isotype: Mouse IgG1, κ