Biolegend, 393009, Brilliant Violet 421™ anti-human CD309 (VEGFR2) Antibody, 25tests

CD309, also known as VEGF-R2, KDR, and Flk-1 (mouse), is a type I transmembrane glycoprotein. It is a member of the CSF-1/PDGF receptor family of type III tyrosine kinase receptors. Human VEGF-R2 is mainly expressed by endothelial cells, embryonic tissues, and megakaryocytes. It plays an important role in the regulation of angiogenesis, vasculogenesis, and vascular permeability. The ligands of VEGF-R2 include VEGF-A, VEGF-C, and VEGF-D splice isoforms. Activation of VEGF-R2 with its ligands results in the receptor dimerization and autophosphorylation, stimulating endothelial cell proliferation and migration.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant Human VEGFR2-Fc Chimera
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: This clone does not cross-react with mouse endothelial cells.
Product Citations: Tamai K, et al. 2022. Cell Rep Methods. 2:100314. PubMed
RRID: AB_2832739 (BioLegend Cat. No. 393009) AB_2832740 (BioLegend Cat. No. 393010)
Structure: Type I transmembrane glycoprotein, type III tyrosine kinase receptor, CSF-1/PDGF receptor family
Distribution: Endothelial cells, embryonic tissues
Function: VEGFR2 regulates angiogenesis as well as vascular development and permeability.
Ligand/Receptor: VEGF-A, VEGF-C, and VEGF-D splice isoforms
Cell Type: Endothelial cells
Biology Area: Angiogenesis, Cell Biology, Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: DiSalvo J, et al. 1995. J Biol Chem. 270:7717. McColl BK, et al. 2003. J Exp Med. 198:863. Albuquerque RJ, et al. 2009. Nat Med. 15:1023. Guangqi E, et al. 2012. J Biol Chem. 287:3029. Koch S, and Claesson-Welsh. 2012. Cold Spring Harb Perspect Med. 2:a006502. Munaut C, et al. 2012. Plos One. 7:e33475. Wehland M, et al. 2013. Int J Mol Sci. 14:9338.
Gene ID: 3791
UniProt: View information about CD309 on UniProt.org
Clone: A16085H
Regulatory Status: RUO
Other Names: VEGFR2, KDR
Isotype: Mouse IgG1, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.