Biolegend, 394812, APC/Fire™ 810 anti-human CD289 (TLR9) Antibody, 100tests

TLR9 is a member of the toll-like receptor family that aids in recognition of pathogen associated molecular patterns (PAMPs). TLR9 recognizes unmethylated CpG sequences in DNA (ie. intracellular bacteria and DNA viruses). TLR9 signals through the MyD88 pathway leading to inflammatory cytokine production, especially production of type I interferons by pDCs.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: CD289 (TLR9)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 810 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * APC/Fire™ 810 has a maximum excitation of 650 nm and a maximum emission of 810 nm. Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect APC/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page.
Excitation Laser: Red Laser (633 nm)
Application Notes: S16013D clone can be used for both surface and intracellular detection of TLR9. ICFC compatible with both the intracellular flow cytometric staining and True-Nuclear™ transcription buffer set. Does not work for WB (tested on Daudi cell line).
RRID: AB_2927844 (BioLegend Cat. No. 394811) AB_2927844 (BioLegend Cat. No. 394812)
Distribution: TLR9 is expressed by B cells and plasmacytoid dendritic cells (pDC).
Ligand/Receptor: TLR9 recognizes unmethylated CpG sequences in DNA.
Cell Type: B cells, Dendritic cells
Biology Area: Bacterial proteins and Toxins, Immuno-Oncology, Immunology, Innate Immunity
Molecular Family: CD Molecules, Innate Immune Signaling, Toll Like Receptors
Antigen References: Hornung V, et al. 2002. J. Immunol. 168:4531. Eaton-Bassiri A, et al. 2004. Infect. Immun. 72:7202. Krieg, A. 2007. J. Clin. Invest. 117:1184.
Gene ID: 54106
UniProt: View information about CD289 on UniProt.org
Clone: S16013D
Regulatory Status: RUO
Other Names: CD289, TLR9, Toll-like receptor 9
Isotype: Rat IgG2a, κ