Biolegend, 397225, PE/Fire™ 810 anti-human CD38 Antibody, 25tests

CD38 is a 45 kD type II transmembrane glycoprotein also known as T10. It is an ADP-ribosyl hydrolase expressed at variable levels on hematopoietic cells and in some non-hematopoietic tissues (such as brain, muscles, and kidney). In humans, it is expressed at high levels on plasma cells and activated T and B cells. By functioning as both a cyclase and a hydrolase, CD38 mediates lymphocyte activation, adhesion, and the metabolism of cADPR and NAADP. CD31 is the ligand of CD38.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CD38 transfectants
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 810 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect PE/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: S17015F is able to cross-block binding of clones HIT2 and HB-7 also raised against human CD38, but not S17015A based on in-house testing.
Product Citations: Struckman NE, et al. 2024. Biomedicines. :12. PubMed
RRID: AB_2894562 (BioLegend Cat. No. 397225)
Structure: ADP-ribosyl cyclase, ectoenzyme, type II glycoprotein, 45 kD
Distribution: T cells, B cells, NK, myeloid, plasma, and dendritic cells
Function: Ecto-ADP-ribosyl cyclase, calcium signaling, cell activation
Ligand/Receptor: CD31, hyaluronic acid
Cell Type: B cells, Dendritic cells, NK cells, Plasma cells, T cells
Biology Area: Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Ferrero E, et al. 1999. J Leuko Biol. 65:151. Lund F, et al. 1995. Immunol. Today 16:469.
Gene ID: 952
UniProt: View information about CD38 on UniProt.org
Clone: S17015F
Regulatory Status: RUO
Other Names: HB7, T10, ADP-ribosyl cyclase
Isotype: Mouse IgG2a, κ