Biolegend, 397802, Purified anti-human CD166 Antibody, 100μg

CD166, also known as the CD6 ligand or the Activated Leukocyte Cell Adhesion Molecule (ALCAM), is a 100-105 kD transmembrane glycoprotein. It belongs to the Ig superfamily of proteins and expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, and neurons. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently CD166 has also been used as a potential cancer stem cell marker. The antibody reacts with human activated leukocyte cell adhesion molecule (ALCAM).
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human CD166
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Ceccarelli S, et al. 2022. Front Cell Dev Biol. 10:926180. PubMed
RRID: AB_2814379 (BioLegend Cat. No. 397802)
Structure: A type I transmembrane glycoprotein belonging to the Ig superfamily of proteins.
Distribution: CD166 is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, and neurons.
Function: Play an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development.
Ligand/Receptor: CD6
Cell Type: B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area: Cell Biology
Molecular Family: CD Molecules
Antigen References: Aruffo A, et al. 1997. Immunol Today. 18(10):498. Patel DD, et al. 1995. J. Exp. Med. 181:2213. Bowen MA, et al. 1995. J. Exp. Med. 181:1563. Horst D, et al. 2009. Cancer Invest. 8:844-50.
Gene ID: 214
UniProt: View information about CD166 on UniProt.org
Clone: A17193J
Regulatory Status: RUO
Other Names: CD6 ligand, Activated Leukocyte Cell Adhesion Molecule (ALCAM)
Isotype: Mouse IgG2b, κ