Biolegend, 398305, Alexa Fluor® 647 anti-human CD32B/C Antibody, 25tests

CD32B (FCGR2B) and CD32C (FCGR2C) are 40 kDa, type I transmembrane proteins that are members of the Ig superfamily of low-affinity immunoglobulin gamma Fc receptors. CD32B has a cytoplasmic tail that contains an immunoreceptor tyrosine-based inhibition motif (ITIM), while CD32C contains an immunoreceptor tyrosine-based activation motif (ITAM). CD32B and CD32C are low affinity receptor for monomeric IgG but also bind IgG complexes. CD32B and CD32C are expressed on B cells, subsets of monocytes,macrophages and granulocytes, platelets, mast cells, and is a negative regulator of cell activation, proliferation, endocytosis, phagocytosis, and degranulation.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant Human Fc gamma RIIB/C (CD32b/c) Protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: As the extracellular region of CD32B and CD32C are identical, this Ab recognizes both isoforms. Does not crossreact with CD32A.
RRID: AB_2860998 (BioLegend Cat. No. 398305) AB_2860999 (BioLegend Cat. No. 398306)
Structure: Type I transmembrane protein, 40 kDa, member of the Ig superfamily
Distribution: B cells, subsets of monocytes,macrophages and granulocytes, platelets, mast cells.
Function: Negatively regulates cell activation, proliferation, endocytosis, phagocytosis, and degranulation
Ligand/Receptor: Fc region of complex or aggregated immunoglobulins gamma
Cell Type: B cells, Dendritic cells, Granulocytes, Monocytes, Platelets
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Fc Receptors
Antigen References: Bruhns P, et al. 2009. Blood. 113: 3716. Bewarder N, et al. 1996. Mol Cell Biol. 16: 4735. Descours B, et al. 2017. Nature. 543: 564. Tomiyama Y, et al. 1992. Blood. 80: 2261. Indik Z, et al. 1991. J Clin Invest. 88: 1766. Ramsland PA, et al. 2011. J Immunol. 187: 3208. Hogarth PM and Pietersz GA. 2012. Nat Rev Drug Discov. 11: 311. Bournazos S, et al. 2009. J Immunol. 182: 8026. Maxwell KF, et al. 1999. Nat Struct Biol. 6: 437. Sandilands GP, et al. 1997. Immunology. 91: 204. Ghazizadeh S, et al. 1994. J Biol Chem. 269: 8878. Gillis C, et al. 2014. Front Immunol. 5: 254.
Gene ID: 22139103
UniProt: View information about FCGR2B+OR+FCGR2B on UniProt.org
Clone: S18005H
Regulatory Status: RUO
Other Names: FCGR2B, FCgRIIB, FCGR2C, FCRIIC, IGFR2, CD32B, CD32C, FCG2, FCGR2, IGFR2, FcRII-c, CDW32
Isotype: Mouse IgG1, κ