Biolegend, 398502, Purified anti-human Ki-67 Antibody, 100μg

Antigen Ki-67 is a nuclear protein expressed as two isoforms with molecular weights of 395 and 345 kD. Both isoforms contain one forkhead-associated domain and 16 concatenated "Ki-67 repeats," each containing the epitope recognized by the mAb Ki-67. The antigen Ki-67 interacts with Hklp2, hNIFK, and chromobox protein homolog 1, 3, and 5. Ki-67 is required for cell proliferation and its expression is restricted to the phases G 1 , S, G 2 , and M of the cell cycle. This characteristic makes Ki-67 an excellent marker for proliferating cells and is commonly used as one of the prognostic factors in cancer studies. Ki-67 has also been used to study myocyte proliferation after myocardial infarction as well as lymphocyte proliferation during infection, and has been used in neurons of patients with different neuropathologies.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human Ki-67 recombinant protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2820065 (BioLegend Cat. No. 398502)
Structure: Two isoforms with molecular weights of 395 and 345 kD, one forkhead-associated domain, 16 concatenated Ki-67 repeats, located in nucleus
Distribution: Expressed in the phases G1, S, G2, and M of the cell cycle
Function: Required for cell proliferation
Interaction: Chromobox protein homolog 1, 3 and 5, Hklp2, and hNIFK
Biology Area: Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication
Molecular Family: Nuclear Markers
Antigen References: Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987. Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174. Drazen JM. et al. 2001. N. Engl. J. Med. 344:1750. Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295. Nagy Z, et al. 1997. Acta. Neuropathol. 93:294.
Gene ID: 4288
UniProt: View information about Ki-67 on UniProt.org
Clone: W17211A
Regulatory Status: RUO
Other Names: MKI67, Proliferation Marker Protein Ki-67, Antigen Ki67
Isotype: Rat IgG2a, κ