Biolegend, 400155, Alexa Fluor® 647 Mouse IgG1, κ Isotype Ctrl (ICFC) Antibody, 50μg

The MOPC-21 immunoglobulin has unknown specificity. The isotype of this antibody is mouse IgG1, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
50μg
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: µg sizes: 0.5 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The mouse IgG1, κ isotype control solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this mouse IgG1, κ isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, use the isotype control at the same concentration as your primary antibody. Use our Concentration Lookup tool to find the exact concentrations of your lots of product. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA)
Application References(PubMed link indicates BioLegend citation): Carlsten M, et al. 2007. Cancer Res. 67:1317. PubMed Smed-Sörensen A, et al. 2008. Blood 111:5037. PubMed (FA) Bunesmann MM, et al. 2011. Am. J. Respir. Cell. Mol. Biol. Epub. PubMed Matsuyama T, et al. 2005. Infect. Immun. 73:1044. (IF) Correia DV, et al. 2011. Blood 118:992. (FC) PubMed Lian IA, et al. 2011. Placenta. 32:823. PubMed Bufe B, et al. 20015. J Biol Chem. 290:7369. PubMed
Product Citations: Pinon L, et al. 2022. Cell Rep Methods. 2:100335. PubMed Yusuf B, et al. 2021. Sci Rep. 11:3614. PubMed Meng KP, et al. 2020. J Exp Med. 217:00:00. PubMed Svensson A, et al. 2017. PLoS One.. 10.1371/journal.pone.0183268. PubMed Herter JM, et al. 2022. Strahlenther Onkol. Online ahead of print. PubMed Ibuki M, et al. 2019. Regen Ther. 0.551388889. PubMed Pardy RD, et al. 2021. Nat Commun. 12:4051. PubMed Gu C, et al. 2021. Front Immunol. 12:678036. PubMed Foltz L, et al. 2020. Mol Biol Cell. 31:2269. PubMed Ren C, et al. 2022. J Cell Mol Med. 26:1071. PubMed Majedi FS, et al. 2020. Biomaterials. 252:120058. PubMed Wang Z, et al. 2015. Mol Oncol. 9: 1458-1470. PubMed Iwata TN, et al. 2018. Mol Cancer Ther. 17:1494. PubMed Yao C, et al. 2018. Autophagy. 1.854861111. PubMed Yang YK, et al. 2021. Stem Cell Res Ther. 12:156. PubMed Pantelidou C, et al. 2022. NPJ Breast Cancer. 8:102. PubMed Dieter SM, et al. 2021. Cell Rep. 36:109394. PubMed
RRID: AB_2832978 (BioLegend Cat. No. 400155) AB_2832978 (BioLegend Cat. No. 400136)
Gene ID: NA
Clone: MOPC-21
Regulatory Status: RUO
Isotype: Mouse IgG1, κ