Biolegend, 400203, Biotin Mouse IgG2a, κ Isotype Ctrl Antibody, 50μg

The MOPC-173 immunoglobulin has unknown specificity. The isotype of this antibody is mouse IgG2a, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat, and human tissues.
50μg
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The immunoglobulin was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. For flow cytometric staining, use the isotype control at the same concentration as your primary antibody. Use our Concentration Lookup tool to find the exact concentrations of your lots of product.
Application Notes: Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA).
Product Citations: Bunting MD, et al. 2022. Sci Adv. 8:eabk3327. PubMed Richardsen E, et al. 2019. Sci Rep. 9:10662. PubMed Paulsen E, et al. 2017. Cancer Immunol Immunother. 10.1007/s00262-017-2039-2. PubMed Halkias J, et al. 2019. J Clin Invest. 130:3562. PubMed
RRID: AB_326453 (BioLegend Cat. No. 400203) AB_326453 (BioLegend Cat. No. 400204)
Gene ID: NA
Clone: MOPC-173
Regulatory Status: RUO
Isotype: Mouse IgG2a, κ
Q: IgG2a gene is deleted in some mouse strains, which ones are they?
A: IgG2a gene is deleted in C57Bl/6, C57Bl/10, SJL, and NOD mice. It is replaced with IgG2c gene instead.
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.