Biolegend, 400260, Brilliant Violet 421™ Mouse IgG2a, κ Isotype Ctrl Antibody, 100tests

The MOPC-173 immunoglobulin has unknown specificity. The isotype of this antibody is mouse IgG2a, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat, and human tissues.
100tests
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The immunoglobulin was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested ICFC - Verified
Recommended Usage: Each lot of this mouse IgG2a, κ isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, use the isotype control at the same concentration as your primary antibody. Use our Concentration Lookup tool to find the exact concentrations of your lots of product. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA).
Product Citations: Pan R, et al. 2022. Cell. 185:1521. PubMed Grayson KA, et al. 2021. Mol Cancer Ther. 20:833. PubMed Jairaman A, et al. 2022. Elife. 11:. PubMed Oh JH, et al. 2019. Cancer Immunol Res. 0.697222222. PubMed Herter JM, et al. 2022. Strahlenther Onkol. Online ahead of print. PubMed Lu Q, et al. 2021. Immunity. 54(6):1304-1319.e9. PubMed Zhu H, et al. 2021. Cell Metabolism. :. PubMed Grayson KA, et al. 2021. PLoS One. 16:e0246733. PubMed Cui D, et al. 2021. Front Cell Dev Biol. 9:656867. PubMed Okamoto Y, et al. 2021. J Cell Sci. 134:. PubMed Cao Y, et al. 2019. Nat Commun. 10:1280. PubMed Riegel K, et al. 2021. Cell Death Dis. 12:969. PubMed Moudgil A, et al. 2020. Cell. 182(4):992-1008.e21. PubMed Tomita T, et al. 2021. Nat Commun. 12:3655. PubMed Demberg T, et al. 2014. Clin Immunol. 153:264. PubMed
RRID: AB_10895919 (BioLegend Cat. No. 400259) AB_10960144 (BioLegend Cat. No. 400260)
Gene ID: NA
Clone: MOPC-173
Regulatory Status: RUO
Isotype: Mouse IgG2a, κ
Q: IgG2a gene is deleted in some mouse strains, which ones are they?
A: IgG2a gene is deleted in C57Bl/6, C57Bl/10, SJL, and NOD mice. It is replaced with IgG2c gene instead.
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.