Biolegend, 400302, Purified Mouse IgG2b, κ Isotype Ctrl Antibody, 500μg

The MPC-11 immunoglobulin has unknown specificity. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
500μg
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The immunoglobulin was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The immunoglobulin solution should be stored undiluted between 2°C and 8°C
Application: FC - Quality tested ChIP - Verified ICFC, ICC, IHC, IP, WB, FA - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.
Application Notes: Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA).
Application References(PubMed link indicates BioLegend citation): Smed-Sörensen A, et al. 2008. Blood 111:5037. (FA) PubMed Podolin PL, et al. 2008. J. Immunol. 180:7989. (FC) PubMed
Product Citations: Aoki T, et al. 2020. Cancer Sci. 111:2223. PubMed Ding C, et al. 2022. FASEB J. 36:e22468. PubMed Peterson HM, et al. 2022. Adv Sci (Weinh). 9:e2200415. PubMed Smed-Sörensen A, et al. 2008. Blood. 111:5037. PubMed Mastoridis S, et al. 2021. Am J Transplant. 21:2387. PubMed Tanaka Y, et al. 2021. Int J Mol Sci. 22:. PubMed Sawanobori Y, et al. 2021. Histochem Cell Biol. 156:133. PubMed Hartley GP, et al. 2018. Cancer Immunol Res. 6:1260. PubMed Li H, et al. 2020. Front Immunol. 11:1777. PubMed Yu S, et al. 2022. Proc Natl Acad Sci U S A. 119:. PubMed N'Da Konan S, et al. 2022. Retrovirology. 19:4. PubMed Ben Saad A, et al. 2021. Int J Mol Sci. 22:. PubMed Tognarelli S, et al. 2018. Front Immunol. 9:2743. PubMed Obiedat A, et al. 2020. J Mol Med (Berl). 98:135. PubMed Hu X, et al. 2020. Neoplasia. 1.290972222. PubMed Nagy-Baló Z, et al. 2021. PLoS One. 16:e0254853. PubMed Zhong Q, et al. 2018. J Immunol. 200:3913. PubMed Mastoridis S, et al. 2018. Front Immunol. 1.474305556. PubMed
RRID: AB_326481 (BioLegend Cat. No. 400301) AB_326481 (BioLegend Cat. No. 400302)
Gene ID: NA
Clone: MPC-11
Regulatory Status: RUO
Isotype: Mouse IgG2b, κ