Biolegend, 400502, Purified Rat IgG2a, κ Isotype Ctrl Antibody, 500μg

The RTK2758 immunoglobulin reacts with KLH. The isotype of this antibody is rat IgG2a, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
500μg
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Trinitrophenol + KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The immunoglobulin was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested ChIP - Verified ICFC, WB, IP, ICC, IHC, FA - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.
Application Notes: Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), and Functional Assay (FA).
Application References(PubMed link indicates BioLegend citation): Nishimoto H, et al. 2005. Blood 106:4241. Seach N, et al. 2008. J. Immunol. 180:5384. PubMed Jiang P, et al. 1999. J. Biol. Chem. 274:559. (FA)
Product Citations: Seach N, et al. 2008. J Immunol. 180:5384. PubMed Yang AC, et al. 2020. Nature. 583:425. PubMed Gargaro M, et al. 2022. Immunity. 55:1032. PubMed Davis-Knowlton J, et al. 2022. J Vasc Res. 59:261. PubMed Gomes P, et al. 2023. Cell Commun Signal. 21:35. PubMed Peterson HM, et al. 2022. Adv Sci (Weinh). 9:e2200415. PubMed Reglero-Real N, et al. 2021. Immunity. :. PubMed Gopinath A, et al. 2020. J Immunol Methods. 476:112686. PubMed Lanitis E, et al. 2021. J Exp Med. 218:. PubMed Oleinika K, et al. 2018. Nat Commun. 9:684. PubMed Palau V, et al. 2021. Int J Mol Sci. 23:. PubMed Palau V, et al. 2021. Int J Mol Sci. 22:. PubMed Ray HC, et al. 2020. Sci Rep. 10:15808. PubMed Karhadkar TR, et al. 2021. PLoS One. 16:e0245924. PubMed Haase C, et al. 2022. Nat Methods. 19:1622. PubMed Donati Y, et al. 2020. Am J Physiol Lung Cell Mol Physiol. L619:318. PubMed Souriant S, et al. 2019. Cell Rep. 26:3586. PubMed Itoh A, et al. 2019. Front Immunol. 2.198611111. PubMed Murashov MD, et al. 2018. Pharmaceutics. 10:. PubMed Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed Park GY, et al. 2020. Immune Netw. e43:20. PubMed Matsumoto K, et al. 2017. PLoS One. 12(6):e0175406. PubMed Sundararaj K, et al. 2021. Immunology. 162:418. PubMed Samoylenko A, et al. 2021. Sci Rep. 11:19594. PubMed Sharma MD, et al. 2018. Immunity. 48:91. PubMed Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed Lever JM, et al. 2019. JCI Insight. 4:e125503. PubMed Lin A, et al. 2022. Bioeng Transl Med. 7:e10314. PubMed Blaskovic S, et al. 2020. Am J Physiol Lung Cell Mol Physiol. L606:318. PubMed Bonavita E, et al. 2020. Immunity. 1215:53. PubMed Kurashima Y, et al. 2021. Nat Commun. 12:1067. PubMed Palau V, et al. 2021. Int J Mol Sci. 22: . PubMed Lopes N, et al. 2017. EMBO Mol Med. 0.954861111. PubMed
RRID: AB_326523 (BioLegend Cat. No. 400501) AB_326523 (BioLegend Cat. No. 400502)
Gene ID: NA
Clone: RTK2758
Regulatory Status: RUO
Isotype: Rat IgG2a, κ