Biolegend, 400707, PE Rat IgG2c, κ Isotype Ctrl Antibody, 25μg

The isotype of RTK4174 immunoglobulin is rat IgG2c, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
25μg
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Trinitrophenol + KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The immunoglobulin was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: The RTK4174 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The ULEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 400732-400737) as negative control.
Application References(PubMed link indicates BioLegend citation): Ganeshah K, et al. 2012. J. Immunol. 188:594. PubMed D'Agostino PM, et al. 2012. PNAS. 109:6175. PubMed
Product Citations: Jauch-Speer SL, et al. 2022. Elife. 11: . PubMed Gerwing M, et al. 2020. Mol Imaging Biol. 1.959027778. PubMed Zhang L, et al. 2022. Front Physiol. 13:929926. PubMed
RRID: AB_326573 (BioLegend Cat. No. 400707)
Gene ID: NA
Clone: RTK4174
Regulatory Status: RUO
Isotype: Rat IgG2c, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.