Biolegend, 401301, Purified Mouse IgG3, κ Isotype Ctrl Antibody, 50μg

The isotype of MG3-35 immunoglobulin is mouse IgG3,κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
50μg
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Trinitrophenol + KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The immunoglobulin was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The immunoglobulin solution should be stored undiluted between 2°C and 8°C
Application: FC - Quality tested ChIP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.
Application Notes: The MG3-35 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, immunofluorescence microscopy, immunocytochemistry and immunofluorescent staining (surface or intracellular) for flow cytometric analysis. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 401310) as negative control.
Product Citations: Silva MA, et al. 2018. PLoS One. 13:e0196464. PubMed Wallis J, et al. 2020. Eur J Pharm Sci. 152:105456. PubMed Rolle A, et al. 2016. Proc Natl Acad Sci U S A. 10.1073/pnas.1518836113. PubMed Pleiner T, et al. 2018. J Cell Biol. 217:1143. PubMed
RRID: AB_2889135 (BioLegend Cat. No. 401301) AB_2889135 (BioLegend Cat. No. 401302)
Gene ID: NA
Clone: MG3-35
Regulatory Status: RUO
Isotype: Mouse IgG3, κ