Biolegend, 401319, PE Mouse IgG3, κ Isotype Ctrl Antibody, 25μg

The isotype of MG3-35 immunoglobulin is mouse IgG3,κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
25μg
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Trinitrophenol + KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, use the isotype control at the same concentration as your primary antibody. Use our Concentration Lookup tool to find the exact concentrations of your lots of product.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: The MG3-35 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, immunofluorescence microscopy, immunocytochemistry and immunofluorescent staining (surface or intracellular) for flow cytometric analysis. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 401310) as negative control.
Product Citations: Kimura Y, et al. 2020. Stem Cell Research. 72.69236111. PubMed Hu K, et al. 2011. Blood. 117:e109. PubMed Tanaka Y et al. 2018. Stem Cell Res. 31:11-15 . PubMed
RRID: AB_10689634 (BioLegend Cat. No. 401319) AB_10683168 (BioLegend Cat. No. 401320)
Gene ID: NA
Clone: MG3-35
Regulatory Status: RUO
Isotype: Mouse IgG3, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.