Biolegend, 401502, Purified Mouse IgG2a, κ Isotype Ctrl Antibody, 500μg

The isotype of MG2a-53 immunoglobulin is mouse IgG2a,κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
500μg
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Trinitrophenol + KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The immunoglobulin was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The immunoglobulin solution should be stored undiluted between 2°C and 8°C
Application: FC - Quality tested ChIP - Verified ICFC, WB, IP, ICC, IHC, FA - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.
Application Notes: Additional reported applications (for the relevant formats) include: Western blotting, immunoprecipitation, immunocytochemistry, immunohistochemistry, and functional assay. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 401507-401512).
Application References(PubMed link indicates BioLegend citation): Bhattacharyya S, et al. 2007. Am. J. Physiol. Gastrointest Liver Physiol. doi:10.1152/ajpgi.00149. Lee H, et al. 2014. Invest. Ophthalmol. Vis. Sci. 55:2885. PubMed Baker GF, et al. 2014. Cancer Res. 74:5079. PubMed
Product Citations: Frasca D, et al. 2020. Front Immunol. 11:392. PubMed Zhang SY, et al. 2023. Signal Transduct Target Ther. 8:236. PubMed Shah D, et al. 2021. Oncoimmunology. 10:1939601. PubMed Vitry J, et al. 2021. Int J Mol Sci. 22:. PubMed Saleh KK, et al. 2022. iScience. 25:105415. PubMed Ogura H, et al. 2022. Nat Commun. 13:7063. PubMed Baker G, et al. 2014. Cancer Res. 74:5079. PubMed Gu C, et al. 2019. J Immunol. 203:389. PubMed Bradford BM, et al. 2018. Parasite Immunol. 40:e12566. PubMed Lee H, et al. 2014. Invest Ophthalmol Vis Sci . 55:2885. PubMed Wang J, et al. 2020. Cell. 183:1264. PubMed Wallis J, et al. 2020. Eur J Pharm Sci. 152:105456. PubMed Narayan S, et al. 2022. iScience. 25:104518. PubMed Yamada S, et al. 2021. J Tissue Eng. 12:20417314211019375. PubMed Liu PY, et al. 2019. Nat Commun. 3.906944444. PubMed Parra-Sánchez H, et al. 2018. Mol Immunol. 96:1. PubMed Xu L, et al. 2020. Nat Cell Biol. 1162:22. PubMed Ghosh R, et al. 2018. PLoS One. 13:e0194611. PubMed Paré G, et al. 2021. Front Immunol. 12:650808. PubMed Dunham C, et al. 2020. FASEB J. 34:12976. PubMed Zhou J, et al. 2020. Nat Commun. 0.625. PubMed Smith TC, et al. 2022. Skelet Muscle. 12:2. PubMed
RRID: AB_2800437 (BioLegend Cat. No. 401501) AB_2800437 (BioLegend Cat. No. 401502)
Gene ID: NA
Clone: MG2a-53
Regulatory Status: RUO
Isotype: Mouse IgG2a, κ
Q: IgG2a gene is deleted in some mouse strains, which ones are they?
A: IgG2a gene is deleted in C57Bl/6, C57Bl/10, SJL, and NOD mice. It is replaced with IgG2c gene instead.