Biolegend, 401610, PE Mouse IgM, κ Isotype Ctrl Antibody, 100μg

The isotype of MM-30 immunoglobulin is mouse IgM, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
100μg
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Trinitrophenol + KLH
Formulation: test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: µg sizes: 0.2 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested ICFC - Verified
Recommended Usage: Each lot of this mouse IgM, κ isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, use the isotype control at the same concentration as your primary antibody. Use our Concentration Lookup tool to find the exact concentrations of your lots of product.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Western blotting, immunoprecipitation, immunocytochemistry, immunohistochemistry, and functional assay. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 401604) as negative control.
Application References(PubMed link indicates BioLegend citation): Ameres S, et al. 2014. J Immunol. 192:5894. PubMed
Product Citations: Chithanathan K, et al. 2022. Cells. 11: . PubMed van den Biggelaar RHGA, et al. 2020. Front Immunol. 11:141. PubMed Yan L, et al. 2021. Front Cell Neurosci. 15:750373. PubMed Ackermann M, et al. 2022. Nat Protoc. 17:513. PubMed Kuang YL, et al. 2019. Stem Cell Res. 37:101434. PubMed Kobayashi Y, et al. 2020. Int J Oncol. 999:56. PubMed Jürchott K, et al. 2016. PLoS One. 11: 0150812. PubMed Wu L, et al. 2018. Oncol Lett. 15:9507. PubMed Philip DL, et al. 2019. Biomacromolecules. 20:1443. PubMed Steenpass L 2017. Stem Cell Res.. 10.1016/j.scr.2017.07.005. PubMed Headland S, et al. 2014. Sci Rep. 4:5237. PubMed
RRID: AB_2892810 (BioLegend Cat. No. 401611) AB_2892810 (BioLegend Cat. No. 401609)
Gene ID: NA
Clone: MM-30
Regulatory Status: RUO
Isotype: Mouse IgM, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.