Biolegend, 402028, PerCP/Cyanine5.5 Syrian Hamster IgG Isotype Ctrl Antibody, 100μg

The SHG-1 immunoglobulin is of Syrian hamster IgG isotype and was chosen as an isotype control after screening on variety of resting, activated, live, and fixed mouse, rat and human tissues.
100μg
Antibody Type: Monoclonal
Host Species: Syrian Hamster
Immunogen: Trinitrophenol + KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The immunoglobulin was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest. Use our Concentration Lookup tool to find the exact concentrations of your lots of product.
Excitation Laser: Blue Laser (488 nm)
Application Notes: The SHG-1 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays as negative control.
Application References(PubMed link indicates BioLegend citation): Nishimoto H, et al. 2005. Blood. 106:4241.
Product Citations: Zhang M, et al. 2017. Mol Med Rep. 15:4291. PubMed
RRID: AB_3097030 (BioLegend Cat. No. 402027) AB_3097030 (BioLegend Cat. No. 402028)
Gene ID: NA
Clone: SHG-1
Regulatory Status: RUO
Isotype: Syrian Hamster IgG
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.