Biolegend, 405102, FITC Avidin, 500μg

Avidin binds to biotin with high affinity. Avidin-fluorescein isothiocyanate (FITC) is useful for detecting biotinylated antibodies. The excitation of FITC by 488 nm laser light induces a light emission maximum of 520 nm.
500μg
Formulation: 10 mM HEPES, 0.5 M NaCl, pH 8.0, containing 0.08% sodium azide.
Preparation: Avidin is conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: This solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested ICFC - Verified
Recommended Usage: Avidin-FITC is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the recommended use of this reagent is ≤0.125 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Avidin-FITC is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies.
Application References(PubMed link indicates BioLegend citation): Jeon OH, et al. 2007. Throb res. 119:609. PubMed Lin HY, et al. 2013. J. Virol. 87:9064. PubMed
Product Citations: Jeon O, et al. 2007. Thromb Res. 119:609. PubMed Lin H, et al. 2013. J Virol. 87:9064. PubMed Quaranta M, et al. 2011. PLoS One. 6:e23442. PubMed Dalangood S, et al. 2020. Theranostics. 10:10078. PubMed
Gene ID: NA
UniProt: View information about Avidin on UniProt.org
Regulatory Status: RUO
Other Names: Avidin-Fluorescein Isothiocyanate, Av-FITC