Biolegend, 405174, PE/Fire™ 700 Streptavidin, 100μg

Streptavidin is a 52.8 kDa tetrameric protein obtained from Streptomyces avidinii. It binds to biotin with a very high affinity and is one of the strongest interactions in nature with a dissociation constant of 10 -14 mol/L. It is used to detect biotinylated proteins in a wide range of applications.
100μg
Verified Reactivity: Human, Mouse, Rat, All Species
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 700 under optimal conditions.
Concentration: 0.2 mg/mL (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * PE/Fire™ 700 has a maximum excitation of 565 nm and a maximum emission of 695 nm.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Streptavidin-PE/Fire™ 700 is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-PE/Fire™ 700 is 330 kD and Streptavidin alone is 52 kD.
Gene ID: NA
UniProt: View information about Biotin on UniProt.org
Regulatory Status: RUO
Other Names: Streptavidin, SAV, SAV, SAV-PE/Fire™ 700