Biolegend, 405205, PE/Cyanine5 Streptavidin, 100μg

Streptavidin binds to biotin with high affinity. Streptavidin-PE/Cyanine5 is useful for detecting biotinylated antibodies. The excitation of PE/Cyanine5 by 488 nm laser light induces a light emission maximum of 670 nm.
100μg
Verified Reactivity: Human, Mouse, Rat, All Species
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: Streptavidin is conjugated with PE/Cyanine5 under optimal conditions.
Concentration: 0.2 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling: The Streptavidin-PE/Cyanine5 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested ICFC - Verified
Recommended Usage: Each lot of this Streptavidin-PE/Cyanine5 is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular molar mass of streptavidin independent of any additional molecular mass that might be added by the PE/Cyanine5 conjugation. For immunofluorescent staining, the recommended use of this reagent is = 0.125 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Streptavidin-Phycoerythrin/Cyanine5 (PE/Cyanine5) is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-PE/Cyanine5 is 480 kD and Streptavidin alone is 52 kD.
Application References(PubMed link indicates BioLegend citation): Wong KA, et al. 2008. J. Immunol. 15:180. PubMed Qi Q, et al. 2009. Blood PubMed Imada M, et al. 2009. Int immunol. PubMed Sawa Y, et al. 2009. Immunity. 30:447. PubMed Song R, et al. 2010. J. Virol. PubMed Abdel-Latif A, et al. 2010. Exp Hematol. 38:1131. PubMed De Monte L, et al. 2011. J. Exp Med. 208:469. PubMed Xu B, et al. 2012. PLoS One. 7:e44719. PubMed Su ML, et al. 2014. PLoS One. 9:98823. PubMed Chen A, et al. 2014. J Immunol. 193:35. PubMed Soni C, et al. 2014. J Immunol. 193:4400. PubMed Wong EB, et al. 2015. J Immunol. 194:4130. PubMed
Product Citations: Ogishi M, et al. 2023. J Exp Med. 220: . PubMed Muliaditan T, et al. 2021. Cell Rep Med. 2:100457. PubMed Song R, et al. 2010. J Virol. 84:6935. PubMed Ogishi M, et al. 2022. J Exp Med. 219:. PubMed Abdel-Latif A, et al. 2010. Exp Hematol. 38:1131. PubMed Rodriguez K 2008. J Immunol. 180:716. PubMed Pereira JL, et al. 2021. Transl Oncol. 14:101125. PubMed Domeier P, et al. 2016. J Exp Med. 213: 715 - 732. PubMed JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed Soni C, et al. 2014. J Immunol. 193:4400. PubMed Su M, et al. 2014. PLoS One. 9:98823. PubMed Imada M, et al. 2009. Int Immunol. 1.674305556. PubMed Cuenca M, et al. 2016. J Immunol. 196: 726 - 737. PubMed Suliman HB, et al. 2022. iScience. 25:103535. PubMed Xu B, et al. 2012. PLoS One. 7:e44719. PubMed Chen A, et al. 2014. J Immunol. 193:35. PubMed Wong E, et al. 2015. J Immunol. 194:4130. PubMed Qi Q, et al. 2009. Blood. 114:564. PubMed Pennington LF, et al. 2021. Nat Commun. 12:7069. PubMed Chiem K, et al. 2021. Microbiol Spectr. 9:e0160121. PubMed Sawa Y, et al. 2009. Immunity. 30:447. PubMed Monte L, et al. 2011. J Exp Med. 208:469. PubMed Li ZG, et al. 2020. Chin Med J (Engl). 1943:133. PubMed Hayashi K, et al. 2020. Nat Commun. 4.832638889. PubMed
Gene ID: NA
UniProt: View information about Biotin on UniProt.org
Regulatory Status: RUO
Other Names: SAv-PE/Cyanine5