Biolegend, 405209, Cyanine5 Streptavidin, 100μg

Streptavidin binds to biotin with high affinity. Streptavidin-Cyanine5 is useful for detecting biotinylated antibodies. The excitation of Cyanine5 by 625-650 nm laser light induces a fluorescence emission maximum of 670 nm.
100μg
Verified Reactivity: Human, Mouse, Rat, All Species
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: Streptavidin is conjugated with Cyanine5 under optimal conditions.
Concentration: 0.5 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling: The streptavidin-Cyanine5 solution should be stored undiluted between 2°C and 8°C, and protected from exposure to light. Do not freeze.
Application: FC - Quality tested ICFC - Verified
Recommended Usage: Each lot of this Streptavidin-Cyanine5 is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Cyanine5 conjugation. For immunofluorescent staining, the recommended use of this reagent is = 0.5 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Streptavidin-Cyanine5 is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of streptavidin-Cyanine5 is 60 kD and Streptavidin alone is 52 kD.
Application References(PubMed link indicates BioLegend citation): Hsu HC, et al. 2007. J. Immunol. 178:5357. Biegler KA, et al. 2012. Cancer Prev Res (Phila). 5:1173. PubMed Cheng JY, et al. 2014. Cancer Res. 74:6856. PubMed
Product Citations: Garrison MA, et al. 2023. Sci Data. 813:10. PubMed Müller-Durovic B, et al. 2016. J Immunol. 197: 2891 - 2899. PubMed Cheng J, et al. 2014. Cancer Res. 74:6856. PubMed Dedeoglu B, et al. 2016. PLoS One. 11: 0150826. PubMed Biegler K, et al. 2012. Cancer Prev Res. 5:1173. PubMed
Gene ID: NA
UniProt: View information about Biotin on UniProt.org
Regulatory Status: RUO
Other Names: SAv-Cyanine5, SAV