Biolegend, 405213, PerCP Streptavidin, 100μg

Streptavidin binds to biotin with high affinity. Streptavidin-PerCP is useful for detecting biotinylated antibodies. The excitation of PerCP by 488 nm laser light induces a light emission maximum of 675 nm.
100μg
Verified Reactivity: Human, Mouse, Rat, All Species
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: Streptavidin is conjugated with PerCP under optimal conditions.
Concentration: 0.2 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling: The Streptavidin-PerCP solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested ICFC - Verified
Recommended Usage: This streptavidin product is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the PerCP conjugation. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Streptavidin-PerCP is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-PerCP is 120 kD and Streptavidin alone is 52 kD.
Application References(PubMed link indicates BioLegend citation): Mara-Koosham G, et al. 2011. Infect Immun. 79:1770. PubMed Liao G, et al. 2012. Gastroenterology. 142:582. PubMed Smith KA, et al. 2012. Infect Immun. 80:3481. PubMed Li J, et al. 2013. Am J respir Cell Mol Biol. 48:314. PubMed Michael A, et al. 2013. J. Immunol. 190:5534. PubMed Tsiganov EN, et al. 2014. J Immunol. 192:4718. PubMed Tsiantoulas D, et al. 2015. J Lipid Res. 56:440. PubMed
Product Citations: Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed Screnci B, et al. 2022. iScience. 25:105665. PubMed Han X, et al. 2017. Int J Mol Sci. 10.3390/ijms18050942. PubMed González-Tajuelo R, et al. 2017. Sci Rep. 7:41841. PubMed Li J, et al. 2013. Am J Respir Cell Mol Biol. 48:314. PubMed Jones M, et al. 2016. PLoS One. 11: 0157271. PubMed Mara-Koosham G, et al. 2011. Infect Immun. 79:1770. PubMed Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed Cousin N, et al. 2021. Cancer Res. 81:4133. PubMed Tsiganov E, et al. 2014. J Immunol. 192:4718. PubMed Köchl R, et al. 2020. Elife. 9:00. PubMed Liao G, et al. 2012. Gastroenterology. 142:582. PubMed Sá da Bandeira D, et al. 2022. Cell Rep. 40:111114. PubMed Tsiantoulas D, et al. 2015. J Lipid Res. 56:440. PubMed Klein H, et al. 2016. J Neuropathol Exp Neurol. 75: 1031 - 1047. PubMed Michel A, et al. 2013. J Immunol. 90:5534. PubMed Luo H, et al. 2019. Cell Rep. 26:945. PubMed Xu G, et al. 2015. Free Radic Biol Med. 87: 15-25. PubMed Hauser J, et al. 2016. Mol Immunol. 80:78-90. PubMed Smith K, et al. 2012. Infect Immun . 80:3481. PubMed Seong J, et al. 2018. Development. 145:14. PubMed
Gene ID: NA
UniProt: View information about Biotin on UniProt.org
Regulatory Status: RUO
Other Names: SAv-PerCP
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.