Biolegend, 405214, PerCP/Cyanine5.5 Streptavidin, 100μg

Streptavidin binds to biotin with high affinity. Streptavidin-PerCP/Cyanine5.5 is useful for detecting biotinylated antibodies. The excitation of PerCP/Cyanine5.5 by 488 nm laser light induces a light emission maximum of 690 nm.
100μg
Verified Reactivity: Human, Mouse, Rat, All Species
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: Streptavidin is conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling: The Streptavidin-PerCP/Cyanine5.5 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested ICFC - Verified
Recommended Usage: Each lot of this Streptavidin-PerCP/Cyanine5.5 is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the PerCP/Cyanine5.5 conjugation. For immunofluorescent staining, the recommended use of this reagent is = 0.06 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Streptavidin-PerCP/Cyanine5.5 is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-PerCP/Cyanine5.5 is 110 kD and Streptavidin alone is 52 kD.
Application References(PubMed link indicates BioLegend citation): Killeen ME, et al. 2013. J. Immunol. 190:4324. PubMed Grage-Griebenow E, et al. 2014. Mol Oncol. 8:982. PubMed Mise-Omata S, et al. 2014. Int Immunol. 26:607. PubMed
Product Citations: Suzuki M, et al. 2015. J Immunol. 195: 1273-1281. PubMed Feng X, et al. 2023. Nat Commun. 14:3208. PubMed Li X, et al. 2022. Haematologica. 107:2576. PubMed Koizumi M, et al. 2022. J Biol Chem. 298:102506. PubMed Tanaka T, et al. 2023. Nat Immunol. 24:439. PubMed Grage-Griebenow E, et al. 2014. Mol Oncol. 8:982. PubMed Zakeri A, et al. 2021. J Extracell Vesicles. 10:e12131. PubMed Hirano KI, et al. 2021. eLife. 0.416666666666667. PubMed Pease NA, et al. 2021. Cell Reports. 34(12):108888. PubMed Greenblatt SM, et al. 2018. Cancer Cell. 33:1111. PubMed Ng KK et al. 2018. eLife. 7 pii: e37851. PubMed Perelman SS, et al. 2021. Nat Microbiol. 6:731. PubMed Dong S, et al. 2021. Nature. 591:117. PubMed Glaros V, et al. 2021. Immunity. 54:2005. PubMed Fournier M, Bijl C 2015. Stem Cells Dev. 24: 2413-2422. PubMed Delacher M, et al. 2020. Immunity. 52:295. PubMed Wittner J, et al. 2022. Mucosal Immunol. 15:668. PubMed Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed Verheijen M, et al. 2020. Cell Rep. 108376:33. PubMed Yuan X, et al. 2017. Elife. 6:e29540. PubMed Fujii T, et al. 2021. Bone Res. 9:4. PubMed Mise-Omata S, et al. 2014. Int Immunol. 26:607. PubMed Pi J, et al. 2022. J Nanobiotechnology. 20:36. PubMed Lopes N, et al. 2022. Elife. 11:. PubMed Wagner AK, et al. 2022. iScience. 25:105137. PubMed Yoshinaga M, et al. 2022. Nat Commun. 13:6435. PubMed Killeen M, et al. 2013. J Immunol. 190:4324. PubMed Li X, et al. 2019. Stem Cells. 37:937. PubMed Furusawa J, et al. 2016. PLoS Pathog. 12: 1005507. PubMed Boguslawski KM, et al. 2020. Sci Adv. 6:eaax7515. PubMed
RRID: AB_2716577 (BioLegend Cat. No. 405214)
Gene ID: NA
UniProt: View information about Biotin on UniProt.org
Regulatory Status: RUO
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.