Biolegend, 405250, APC/Fire™ 750 Streptavidin, 100μg

Streptavidin binds to biotin with high affinity. Streptavidin-APC/Fire™ 750 is useful for detecting biotinylated antibodies. The excitation of APC/Fire™ 750 by 600-635 nm laser light induces a fluorescence emission maximum of 787 nm.
100μg
Verified Reactivity: Human, Mouse, Rat, All Species
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: Streptavidin is conjugated with APC/Fire™ 750 under optimal conditions.
Concentration: 0.2 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling: The Streptavidin APC/Fire™ 750 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested ICFC - Verified
Recommended Usage: Each lot of this Streptavidin APC/Fire™ 750 is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the APC/Fire™ 750 conjugation. For immunofluorescent staining, the recommended use of this reagent is ≤0.25 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application. *APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.
Excitation Laser: Red Laser (633 nm)
Application Notes: Streptavidin-Allophycocyanin/Fire™ 750 (APC/Fire™ 750 ) is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-APC/Fire™ 750 is 210 kD and Streptavidin alone is 52 kD.
Application References(PubMed link indicates BioLegend citation): Maresz K, et al. 2005. J. Neurochemistry 95:437. Ponomarev ED, et al. 2006. J. Immunol. 176:1402.PubMed. Ponomarev ED, et al. 2007. J. Immunol. 178:39. Mann MK, et al. 2007. J. Immunol. 178:3447.PubMed Ponomarev ED,et al.2005.J. Immunol Methods.300:32. PubMed Cao YA, et al. 2008. Blood PubMed Garidou L, et al. 2009. J. Virol. PubMed Dolgachev V, et al. 2009. J. Immunol. 183:5705. PubMed Sountoulidis A, et al. 2012. PLoS One. 7:e41460. PubMed. Izumi T, et al. 2013. Cytotherapy. 15:481. PubMed Hamanaka S, et al. 2013. Biochem Biophys Res Commun. 435:586. PubMed Prince J, et al. 2013. J. Immunol. 191:4709. PubMed Phanse Y, et al. 2013. Acta Biomater. 9:8902. PubMed Orre M, et al. 2014. Neurbiol Aging. 35:1. PubMed Barbera M, et al. 2014. Gut. 64:11. PubMed Zhang Y, et al. 2014. Infect Immun. 82:1698. PubMed Peterson BC, et al. 2014. J Leukoc Biol. 95:809. PubMed Barbera M, et al. 2015. Gut. 64:11. PubMed Charmsaz S, et al. 2015. PLoS One. 10:130692. PubMed
Product Citations: Chen GD, et al. 2023. Sci Adv. 9:eabo7605. PubMed Mudd PA, et al. 2022. Cell. 185:603. PubMed
Gene ID: NA
UniProt: View information about Biotin on UniProt.org
Regulatory Status: RUO
Other Names: Streptavidin-Allophycocyanin, SAv-APC