Biolegend, 405308, APC Goat anti-mouse IgG (minimal x-reactivity) Antibody, 100μg

This polyclonal goat anti-mouse IgG antibody reacts with the heavy chains of mouse IgG and with the light (kappa and lambda) chains common to most mouse immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and rabbit immunoglobulins, but it may have minimal cross-reactivity with other subclasses of mouse immunoglobulins.
100μg
Verified Reactivity: Mouse
Antibody Type: Polyclonal
Host Species: Goat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: This polyclonal goat anti-mouse IgG antibody is useful for capture or detection of mouse IgG in ELISA.
Application References(PubMed link indicates BioLegend citation): Scotta C, et al. 2008. J Immunol. 181:1025-33. PubMed Gao X, et al. 2014. Cell Mol Neurobiol. 34:257-68 (ICC) Winters T, et al. 2014. EMBO J. 33:1256-70.
Product Citations: Gusscott S, et al. 2016. PLoS One. 11: 0161158. PubMed Ecker JW, et al. 2020. Vaccines (Basel). 8:00. PubMed Daradoumis J, et al. 2023. Viruses. 15:. PubMed Nielsen OL, et al. 2022. APMIS. 130:359. PubMed Zhang JZ, et al. 2022. Nat Biotechnol. 40:1336. PubMed Liu H, et al. 2022. Cell Rep Med. 3:100660. PubMed Mamula D, et al. 2022. NPJ Parkinsons Dis. 8:171. PubMed Bordoloi D, et al. 2022. JCI Insight. 7: . PubMed Kodali S, et al. 2022. J Immunol. 208:1085. PubMed Uhlenbrock F, et al. 2014. J Immunol. 193:1654. PubMed Muliaditan T, et al. 2021. Cell Rep Med. 2:100457. PubMed Ercoli G, et al. 2021. Front Immunol. 11:611661. PubMed Robinson S, et al. 2015. Virus Res. 203:56. PubMed Miao X, et al. 2020. MAbs. 12:1804241. PubMed Baniel CC, et al. 2020. Front Immunol. 1.576388889. PubMed Shmuel-Galia L, et al. 2017. J Biol Chem. 292:13415. PubMed Zhang H, et al. 2017. Virol Sin. 32:503. PubMed Siddiqui SS, et al. 2021. Proc Natl Acad Sci U S A. 118: . PubMed Tonouchi K, et al. 2020. Int Immunol. 613:32. PubMed Neukirch L, et al. 2019. Oncotarget. 10:1458. PubMed Mellergaard M, et al. 2014. J Biol Chem. 289:20078. PubMed Mellergaard M, et al. 2020. J Biol Chem. 295:11803. PubMed Lee A, et al. 2014. Vet J. 200:103. PubMed Schober T, et al. 2017. Nat Commun. 8:14209. PubMed Møller SH, et al. 2020. Front Immunol. 1.825. PubMed Ge J, et al. 2021. Nat Commun. 0.673611111. PubMed Wang J et al. 2018. Cell. 176(1-2):334-347 . PubMed Shlapobersky M, et al. 2012. J Gen Virol. 93:1305. PubMed Høgh RI, et al. 2020. J Immunol. 1746:204. PubMed González-Tajuelo R, et al. 2020. Front Immunol. 11:588212. PubMed Moogk D, et al. 2016. J Immunol. 197: 644 - 654. PubMed Lukas S, et al. 2013. J Biomol Screen. 19:407. PubMed Denies S, et al. 2014. J Control Release. 187:175. PubMed Noah AC, et al. 2020. J Appl Physiol (1985). 473:128. PubMed Kinchen J et al. 2018. Cell. 175(2):372-386 . PubMed Satchwell T, et al. 2016. Haematologica. 101: 1018 - 1027. PubMed Chandrasekaran S, et al. 2014. PLoS One. 9:111487. PubMed Kask A, et al. 2010. Vaccine. 28:7483. PubMed Campbell C et al. 2018. Immunity. 48(6):1245-1257 . PubMed Mao D, et al. 2022. iScience. 25:103843. PubMed Tsao LC, et al. 2022. JCI Insight. . PubMed Ercoli G, et al. 2022. Clin Transl Immunology. 11:e1366. PubMed Capelle CM, et al. 2021. iScience. 24:102289. PubMed Bruveris FF, et al. 2020. Development. 147:00:00. PubMed Wang Q, et al. 2020. Cell. 181(4):894-904.e9.. PubMed Ardolino M, et al. 2011. Blood. 117:4778. PubMed Wittner J, et al. 2022. Mucosal Immunol. 15:668. PubMed Sauter K, et al. 2016. Am J Physiol Gastrointest Liver Physiol. 311: G533 - G547. PubMed Kawamura A, et al. 2016. Sci Rep. 6:19464. PubMed Liu K, et al. 2020. EMBO Rep. 21:e51444. PubMed Miki K, et al. 2021. Nat Commun. 12:3596. PubMed Uno N, et al. 2015. Mol Ther Nucleic Acids. 4, e272. PubMed Wendt E, et al. 2016. J Immunol. 196: 3910 - 3919. PubMed Yao C, et al. 2018. Autophagy. 1.854861111. PubMed Wang X, et al. 2016. J Immunol. 196: 4090 - 4099. PubMed Uhlenbrock F, et al. 2015. Mol Immunol. 66: 418-427. PubMed Luteijn RD, et al. 2019. Nature. 573:434. PubMed Wang H, et al. 2019. Proc Natl Acad Sci U S A. 116:988. PubMed Pezhouman A, et al. 2022. Cardiovasc Res. 118:828. PubMed Sun H, et al. 2016. Nat Commun. 7:10339. PubMed Ding S, et al. 2016. PLoS Pathog. 12:e1005929. PubMed Wang X, et al. 2020. Immunity. 328:52. PubMed Chen RE, et al. 2021. Nat Med. 717:27. PubMed Tiwarekar V, et al. 2018. J Virol. 92:17. PubMed Tam K, et al. 2016. MBio. 7: e02024-16. PubMed Giri T, et al. 2022. Heliyon. 8:e09407. PubMed Jang A, et al. 2021. Front Immunol. 12:701066. PubMed
RRID: AB_315011 (BioLegend Cat. No. 405308)
Distribution: B cells
Gene ID: 16059
UniProt: View information about IgG on UniProt.org
Clone: Poly4053
Regulatory Status: RUO
Isotype: Goat Polyclonal IgG