Biolegend, 405319, Alexa Fluor® 488 Goat anti-mouse IgG (minimal x-reactivity) Antibody, 100μg

This polyclonal goat anti-mouse IgG antibody reacts with the heavy chains of mouse IgG and with the light (kappa and lambda) chains common to most mouse immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and rabbit immunoglobulins, but it may have minimal cross-reactivity with other subclasses of mouse immunoglobulins.
100μg
Verified Reactivity: Mouse
Antibody Type: Polyclonal
Host Species: Goat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested IHC-F - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application Notes: This polyclonal goat anti-mouse IgG antibody is useful for capture or detection of mouse IgG in ELISA.
Application References(PubMed link indicates BioLegend citation): Scotta C, et al. 2008. J Immunol. 181:1025-33. PubMed Gao X, et al. 2014. Cell Mol Neurobiol. 34:257-68 (ICC) Winters T, et al. 2014. EMBO J. 33:1256-70.
Product Citations: Frasca D, et al. 2020. Front Immunol. 11:392. PubMed Moore ER, et al. 2021. Bone. 143:115738. PubMed Chandler A, et al. 2022. J Orthop Res. 40:1918. PubMed Brown CW, et al. 2020. Developmental Cell. 51(5):575-586.e4.. PubMed Rahimipour M, et al. 2021. Cell J. 23:154. PubMed Chen BR, et al. 2022. Methods Mol Biol. 2444:15. PubMed Dhande IS, et al. 2020. J Am Heart Assoc. 9:e014142. PubMed Varea O, et al. 2022. Brain Commun. 4:fcac168. PubMed Alberts A, et al. 2020. Sci Rep. 10:6391. PubMed Trapecar M, et al. 2021. Sci Adv. 7:00. PubMed Liu Y, et al. 2022. J Inflamm Res. 15:4315. PubMed Tomić S, et al. 2017. Biomaterials. 10.1016/j.biomaterials.2017.08.040. PubMed Wennerberg E, et al. 2022. Sci Transl Med. 14:eabe8195. PubMed Duran J, et al. 2020. Mol Neurobiol. 57:4657. PubMed Bianchi E, et al. 2021. Nat Commun. 12:1251. PubMed Slabicki M, et al. 2020. Nature. 588:164. PubMed Kim J, et al. 2022. Sci Rep. 12:7753. PubMed Wu H, et al. 2016. Sci Rep. 6:28065. PubMed Trapecar M, et al. 2020. Cell Systems. 10(3):223-239. PubMed Fu W, et al. 2021. Front Immunol. 12:656090. PubMed Zelenova EA, et al. 2021. Cells. 10:. PubMed Varea O, et al. 2021. Neurobiol Dis. 147:105173. PubMed Alberts A, et al. 2020. Front Immunol. 11:596103. PubMed Wang X, et al. 2020. Immunity. 328:52. PubMed Nie Y, et al. 2021. Virol Sin. 36:692. PubMed Bidet K, et al. 2019. NPJ Vaccines. 4:27. PubMed
RRID: AB_2563044 (BioLegend Cat. No. 405319)
Distribution: B cells
Gene ID: 16059
UniProt: View information about IgG on UniProt.org
Clone: Poly4053
Regulatory Status: RUO
Isotype: Goat Polyclonal IgG