Biolegend, 405388, PE/Fire™ 744 Streptavidin, 100μg

Streptavidin is a 52.8 kD tetrameric protein obtained from Streptomyces avidinii. It binds to biotin with a very high affinity and is one of the strongest interactions in nature with a dissociation constant of 10 -14 mol/L. It is used to detect biotinylated proteins in a wide range of applications.
100μg
Verified Reactivity: Human, Mouse, Rat, All Species
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: Streptavidin is conjugated with PE/Fire™ 744 under optimal conditions.
Concentration: 0.2 mg/mL (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling: The Streptavidin PE/Fire™ 744 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: This streptavidin product is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the PE/Fire™ 744 conjugation. For flow cytometric staining, the suggested use of this reagent is ≤ 0.03 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. True-Stain Monocyte Blocker™ (Cat No. 426102) is recommended to minimize non-specific staining of PE/Fire™ 744 on monocytes and macrophages. * PE/Fire™ 744 has a maximum excitation of 565 nm and a maximum emission of 744 nm.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Gene ID: NA
Regulatory Status: RUO
Other Names: Streptavidin- PE/Fire™ 744, SAv- PE/Fire™ 744, streptavidin PE/Fire™ 744