Biolegend, 405405, HRP Goat anti-rat IgG (minimal x-reactivity) Antibody, 500microl

Goat anti-rat IgG antibody reacts primarily with the heavy chains of rat IgG, but also partially binds to the light chains common to most rat immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rabbit, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other immunoglobulins from other species.
500microl
Verified Reactivity: Rat
Antibody Type: Polyclonal
Host Species: Goat
Formulation: Aqueous buffered solution containing a carrier protein and preservatives.
Preparation: The polyclonal antibody was purified from goat antiserum by rat immunoglobulin affinity chromatography.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. To obtain lot-specific expiration date, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)This product has a shelf-life of 12 months or less. Please use our Expiration Lookup Tool to verify the expirations date of your lot of product
Application: ELISA - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. Do not use this product beyond the expiration date. For ELISA or Western blot analysis, the reagent should be titrated between 1:1,000 - 1:5,000 to determine optimal conditions. For IHC on tissue sections, the reagent should be titrated between 1:500 - 1:2000 to determine optimal conditions. It is recommended that the reagent be titrated for optimal performance for each application. Avoid using diluent solutions containing sodium azide. Sodium azide is an inhibitor of horseradish peroxidase.
Application Notes: This goat anti-rat IgG antibody is useful for direct immunofluorescent staining or as a second step reagent for indirect immunofluorescent staining when used in conjunction with primary antibodies.
Additional Product Notes: This HRP conjugated goat anti-rat IgG antibody is useful as a second step reagent for indirect immunohistochemistry staining when used in conjunction with primary antibodies.
Application References(PubMed link indicates BioLegend citation): Zornetzer GA, et al. 2010. J. Virol. 84:11297. PubMed Frank NY, et al. 2011. Cancer Res. 71:1474. (IHC) PubMed Baba T, et al. 2020. eLife. 9:e54257. (IHC-P)
Product Citations: Mamedov T, et al. 2019. PLoS One. 14:e0213438. PubMed Ma Y, et al. 2021. Sci Rep. 11:13471. PubMed Malik TH, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed Wang L, et al. 2022. Viruses. 14: . PubMed Domenger A, et al. 2023. Front Oncol. 13:1110916. PubMed van Tok MN, et al. 2018. Front Immunol. 9:1550. PubMed Baba T, et al. 2021. Cell Death Dis. 12:322. PubMed Domenger A, et al. 2022. EMBO Mol Med. 14:e14740. PubMed Kim DH, et al. 2018. Nat Commun. 9:503. PubMed Mamedov T, et al. 2021. Front Plant Sci. 12:742875. PubMed Sahlberg S, et al. 2014. PLoS One. 9:94621. PubMed Lou Q, et al. 2019. J Immunother Cancer. 7:210. PubMed Snelling T, et al. 2022. Biochem J. :. PubMed Zuo X, et al. 2014. PLoS One. 9:84748. PubMed Lu SW, et al. 2020. Nat Commun. 3.660416667. PubMed Link E, et al. 2017. Vaccine. 10.1016/j.vaccine.2017.08.019. PubMed Callejas BE, et al. 2021. Front Immunol. 12:744738. PubMed Freidl R, et al. 2016. J Allergy Clin Immunol. S0091-6749(16)31348-3. PubMed Tuncel J, et al. 2016. PLoS One. 11: 0155936. PubMed Mamedov T, et al. 2019. Sci Rep. 9:9868. PubMed Hayashi M, et al. 2021. Viruses. 13:. PubMed Siddiqui S, et al. 2016. J Biol Chem. 292:1029-1037. PubMed
RRID: AB_315016 (BioLegend Cat. No. 405405)
Gene ID: 295279
UniProt: View information about IgG on UniProt.org
Clone: Poly4054
Regulatory Status: RUO
Isotype: Goat Polyclonal IgG