Biolegend, 405406, PE Goat anti-rat IgG (minimal x-reactivity) Antibody, 200μg

Goat anti-rat IgG antibody reacts primarily with the heavy chains of rat IgG, but also partially binds to the light chains common to most rat immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rabbit, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other immunoglobulins from other species.
200μg
Verified Reactivity: Rat
Antibody Type: Polyclonal
Host Species: Goat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: This goat anti-rat IgG antibody is useful for direct immunofluorescent staining or as a second step reagent for indirect immunofluorescent staining when used in conjunction with primary antibodies.
Application References(PubMed link indicates BioLegend citation): Zornetzer GA, et al. 2010. J. Virol. 84:11297. PubMed Frank NY, et al. 2011. Cancer Res. 71:1474. (IHC) PubMed Baba T, et al. 2020. eLife. 9:e54257. (IHC-P)
Product Citations: Tran K, et al. 2013. Carcinogenesis. 34:199. PubMed Silva D, et al. 2015. Papillomavirus Res. 1: 12-21. PubMed Dorraji SE, et al. 2022. Cancers (Basel). 14:. PubMed Kihara Y, et al. 2022. FASEB J. 36:e22132. PubMed Mekkaoui L, et al. 2020. J Gen Virol. 1008:101. PubMed Biasco L, et al. 2021. Nat Cancer. 2:629. PubMed Peres-Emidio EC, et al. 2022. Front Cell Infect Microbiol. 12:811474. PubMed Warram J, et al. 2011. J Ultrasound Med. 30:921. PubMed Tsao LC, et al. 2022. JCI Insight. . PubMed Bi E, et al. 2018. J Clin Invest. 128:4821. PubMed Sorace A, et al. 2012. J Ultrasound Med. 31:1543. PubMed Zhang L, et al. 2014. J Immunol. 193:1681. PubMed Bisht K, et al. 2014. Biochem Biophys Res Commun. 449:94. PubMed Edelmann B, et al. 2018. J Clin Invest. 128:4359. PubMed Kobayashi F, et al. 2011. Infect Immun. 79:4791. PubMed
RRID: AB_315017 (BioLegend Cat. No. 405406)
Gene ID: 295279
UniProt: View information about IgG on UniProt.org
Clone: Poly4054
Regulatory Status: RUO
Isotype: Goat Polyclonal IgG
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.