Biolegend, 405418, Alexa Fluor® 488 Goat anti-rat IgG (minimal x-reactivity) Antibody, 100μg

Goat anti-rat IgG antibody reacts primarily with the heavy chains of rat IgG, but also partially binds to the light chains common to most rat immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rabbit, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other immunoglobulins from other species.
100μg
Verified Reactivity: Rat
Antibody Type: Polyclonal
Host Species: Goat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested IHC-F - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application Notes: This goat anti-rat IgG antibody is useful for direct immunofluorescent staining or as a second step reagent for indirect immunofluorescent staining when used in conjunction with primary antibodies.
Application References(PubMed link indicates BioLegend citation): Zornetzer GA, et al. 2010. J. Virol. 84:11297. PubMed Frank NY, et al. 2011. Cancer Res. 71:1474. (IHC) PubMed Baba T, et al. 2020. eLife. 9:e54257. (IHC-P)
Product Citations: Chen BR, et al. 2022. Bio Protoc. 12:e4413. PubMed Wang JX, et al. 2023. JCI Insight. 8:. PubMed Koop K, et al. 2023. Front Immunol. 14:1163198. PubMed Fowler FC, et al. 2022. Elife. 11: . PubMed Jeon EY, et al. 2023. Bioeng Transl Med. 8:e10362. PubMed Song JW, et al. 2021. Theranostics. 11:8874. PubMed Bowers E, et al. 2018. Nat Med. 24:95. PubMed Coleman LG, et al. 2018. Brain Behav Immun. 72:61. PubMed Garcia GL, et al. 2020. Am J Pathol. 543:190. PubMed Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed Lisk C, et al. 2020. Front Immunol. 1.329166667. PubMed Blackshear CP, et al. 2018. Tissue Eng Part C Methods. 24:566. PubMed Chen BR, et al. 2021. Elife. 10:. PubMed Desbien A, et al. 2016. J Immunol. 197:4351-4359. PubMed Mead BE et al. 2018. BMC biology. 16(1):62 . PubMed Huang L, et al. 2016. Cell Death Dis. 7:e2301. PubMed Naito H, et al. 2019. Dev Cell. 48:151. PubMed
RRID: AB_2563120 (BioLegend Cat. No. 405418)
Gene ID: 295279
UniProt: View information about IgG on UniProt.org
Clone: Poly4054
Regulatory Status: RUO
Isotype: Goat Polyclonal IgG