Biolegend, 405702, Purified anti-mouse IgD Antibody, 500μg

Surface IgD is an important B cell differentiation marker.
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality testedIHC-P, IHC-F, ICC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The 11-26c.2a antibody reacts with immunoglobulin D in all tested mouse haplotypes. The antibody binds membrane IgD expressed on most B cells. The 11-26c.2a antibody neither induces proliferation of splenic B cells nor induces B cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections2,3, and spatial biology (IBEX)10,11.
Application References(PubMed link indicates BioLegend citation): Nitschke L, et al. 1993. P. Natl. Acad. Sci. USA 90:1887. (FC) Weih D, et al. 2001. J. Immunol. 167:1909. (IHC) Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC) Ahuja A, et al. 2007. J. Immunol. 179:3351. (FC) PubMed Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC) Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed Tomayko MM, et al. 2010. J. Immunol. 185:7146. PubMed Park SY, et al. 2013. J. Immunol. 190:1094. PubMed Rouaud P, et al. 2014. J Exp Med. 211:975. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Avalos A, et al. 2022. JCI Insight. 7: . PubMed Campisi L, et al. 2022. Nature. 606:945. PubMed Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed Brooks JF, et al. 2020. Cytotherapy. 22:436. PubMed Niss Arfelt K, et al. 2017. Blood. 129:866. PubMed van Dinther D, et al. 2019. Cancers (Basel). 0.585416667. PubMed Ahuja A, et al. 2007. J Immunol. 179:3351. PubMed Zhong X, et al. 2020. Proc Natl Acad Sci U S A. 8563:117. PubMed Park S, et al. 2013. J Immunol. 190:1094. PubMed Majer O, et al. 2019. Nature. 575:366. PubMed Zelazowska MA, et al. 2020. Life Sci Alliance. :3. PubMed King IL, et al. 2017. Mucosal Immunol. 10:1160. PubMed Englezou PC, et al. 2018. Mol Ther Nucleic Acids. 12:118. PubMed Tomayko M, et al. 2010. J Immunol. 185:7146. PubMed Paun A, et al. 2021. Blood Adv. 5:745. PubMed
RRID: AB_315024 (BioLegend Cat. No. 405702)
Structure: Ig family
Distribution: B cells
Function: B cell differentiation
Cell Type: B cells
Biology Area: Immunology
Gene ID: 380797
UniProt: View information about IgD on UniProt.org
Clone: 11-26c.2a
Regulatory Status: RUO
Other Names: Immunoglobulin D
Isotype: Rat IgG2a, κ