Biolegend, 405710, PerCP/Cyanine5.5 anti-mouse IgD Antibody, 100μg

Surface IgD is an important B cell differentiation marker.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: The 11-26c.2a antibody reacts with immunoglobulin D in all tested mouse haplotypes. The antibody binds membrane IgD expressed on most B cells. The 11-26c.2a antibody neither induces proliferation of splenic B cells nor induces B cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections2,3, and spatial biology (IBEX)10,11.
Application References(PubMed link indicates BioLegend citation): Nitschke L, et al. 1993. P. Natl. Acad. Sci. USA 90:1887. (FC) Weih D, et al. 2001. J. Immunol. 167:1909. (IHC) Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC) Ahuja A, et al. 2007. J. Immunol. 179:3351. (FC) PubMed Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC) Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed Tomayko MM, et al. 2010. J. Immunol. 185:7146. PubMed Park SY, et al. 2013. J. Immunol. 190:1094. PubMed Rouaud P, et al. 2014. J Exp Med. 211:975. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Knudsen ML, et al. 2022. iScience. 25:105473. PubMed Hutter K, et al. 2022. Front Immunol. 13:967914. PubMed Guilbert C, et al. 2020. Curr Protoc Toxicol. 83:e91. PubMed Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed Kelly A, et al. 2013. Toxicol Sci. 131:434. PubMed Lin YR, et al. 2020. Immunity. 53(4):840-851.e6. PubMed Turner JS et al. 2018. Cell reports. 25(6):1395-1403 . PubMed Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed Komban RJ, et al. 2019. Nat Commun. 10:2423. PubMed Na B, et al. 2016. PLoS One. 11: 0156429. PubMed Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed Parks KR, et al. 2019. Cell Rep. 29:3060. PubMed Wong R, et al. 2020. Immunity. 53(5):1078-1094.e7. PubMed Torres-Ruiz J, et al. 2021. Front Immunol. 12:689966. PubMed Duan L, et al. 2021. Immunity. 54:2256. PubMed Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed Hutter K, et al. 2020. FEBS J. . PubMed Zhu Y, et al. 2022. Clin Transl Med. 12:e887. PubMed Knizkova D, et al. 2022. Nat Immunol. 23:1644. PubMed Bemark M, et al. 2016. Nat Commun. 7: 12698. PubMed Chauveau L, et al. 2021. EMBO Rep. 22:e52447. PubMed Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed He Z, et al. 2016. Exp Hematol. 44:161-165. PubMed Frost JN, et al. 2021. Med (N Y). 2:164. PubMed Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed Yin S et al. 2019. Cancer cell. 35(2):283-296 . PubMed Cantor DJ et al. 2019. Cell reports. 26(1):108-118 . PubMed Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed Sabouri Z, et al. 2016. Nat Commun. 7:13381. PubMed Prüss H, et al. 2017. Nat Neurosci. 10.1038/nn.4643. PubMed Monzon-Casanova E, et al. 2021. Eur J Immunol. 51:2266. PubMed Jing Y, et al. 2019. J Allergy Clin Immunol. 144:1377. PubMed Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed
RRID: AB_1575115 (BioLegend Cat. No. 405709) AB_1575113 (BioLegend Cat. No. 405710)
Structure: Ig family
Distribution: B cells
Function: B cell differentiation
Cell Type: B cells
Biology Area: Immunology
Gene ID: 380797
UniProt: View information about IgD on UniProt.org
Clone: 11-26c.2a
Regulatory Status: RUO
Other Names: Immunoglobulin D
Isotype: Rat IgG2a, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.